Rapid pre‐concentration of Escherichia coli in a microfluidic paper‐based device using ion concentration polarization

• Published in: Electrophoresis Vol. 41; no. 10-11; pp. 867 - 874
• Main Authors: Perera, A.T.K., Pudasaini, Sanam, Ahmed, Syed Shaheer Uddin, Phan, Dinh‐Tuan, Liu, Yu, Yang, Chun
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.06.2020
• Subjects: Coastal waters; E coli; Electrodes; Environmental monitoring; Equipment Design; Escherichia coli; Escherichia coli - isolation & purification; Fluorescence; Fluorocarbon Polymers - chemistry; Ion concentration; Ion concentration polarization; Ions; Ions - chemistry; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Microfluidic paper based analytical device; Microfluidics; Polarization; Pre‐concentration; Sodium Chloride - chemistry; Water Microbiology; Water quality; Microfluidic paper based analytical device; Ion concentration polarization; Pre-concentration; Escherichia coli
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.201900303

We report a microfluidic paper based analytical device implementing ion concentration polarization (ICP) for rapid pre‐concentration of Escherichia coli in water. The fabricated device consists of a paper channel with a Nafion® membrane and in‐built micro wire electrodes to supply electric voltage to induce the ICP effect. E. coli cells were stained with SYTO 9 and fluorescence was used as a sensing method. The device achieved high concentration factor up to 2 × 105 within minutes. The effect of total ion concentration, on ICP and fluorescence intensity was studied. The reported device and method are suitable and effective for detection of E. coli during ballast water quality monitoring, coastal water quality monitoring where high salinity water is present.