Complexation of Fatty Acids and Fatty Acid-CoAs with Synthetic O-Methylated Polysaccharides

• Published in: Chembiochem : a European journal of chemical biology Vol. 8; no. 3; pp. 353 - 359
• Main Authors: Cheon, Hwan-Sung, Wang, Yonghui, Ma, Jianguo, Kishi, Yoshito
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 12.02.2007; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: Acyl Coenzyme A - chemistry; aggregation; fatty acids; Fatty Acids - chemistry; Fluorescent Dyes - chemistry; host-guest systems; hydrophobic effect; Methylmannosides - chemistry; Molecular Structure; oligosaccharides; Polysaccharides - chemistry
• ISSN: 1439-4227; 1439-7633
• DOI: 10.1002/cbic.200600499

An experimentally simple, but highly reproducible and reliable method has been developed to follow the complexation event of fatty acid (FA) and FA-CoA with polysaccharides. This method was based on the recent discovery of the unique blue-shifted UV absorption associated with the aggregation of tetraenoic fatty acids (TE-FAs) in aqueous solution. Complexation was monitored by recording the intensity of UV absorption at 250 nm ([free TE-FA]) and 303 nm ([complexed TE-FA]), and the K aggregate of C₂₀ t,t,t,t-TE-FA exhibited the ideal property for this purpose. Synthetic 3-O-methyl-D-mannose- and 6-O-methyl-D-glucose-containing lipopolysaccharides were found to exhibit a broad range of the binding affinities with C₂₀ t,t,t,t-TE-FAs as well as saturated FAs/FA-CoAs.