Angiotensin-(1-7), the product of ACE2 ameliorates NAFLD by acting through its receptor Mas to regulate hepatic mitochondrial function and glycolipid metabolism

• Published in: The FASEB Journal Vol. 34; no. 12; p. 16291
• Main Authors: Song, Li-Ni, Liu, Jing-Yi, Shi, Ting-Ting, Zhang, Yi-Chen, Xin, Zhong, Cao, Xi, Yang, Jin-Kui
• Format: Journal Article Web Resource
• Language: English
• Published: United States John Wiley & Sons, Inc 01.12.2020
• Subjects: Angiotensin I - metabolism; Angiotensin-Converting Enzyme 2 - metabolism; Animals; Cell Line, Tumor; Down-Regulation - physiology; Glycolipids - metabolism; Hep G2 Cells; Humans; Insulin Resistance - physiology; Lipid Metabolism - physiology; Liver - metabolism; Liver cirrhosis; Male; Mice; Mice, Inbred C57BL; Mitochondria - metabolism; Non-alcoholic Fatty Liver Disease - metabolism; Peptide Fragments - metabolism; Proto-Oncogene Proteins - metabolism; Receptors, G-Protein-Coupled - metabolism; Signal Transduction - physiology; ACE2 | Ang-(1-7); mitochondrial function; glucose metabolism; Mas; hepatic steatosis
• ISSN: 1530-6860
• DOI: 10.1096/fj.202001639R

Nonalcoholic fatty liver disease (NAFLD) is the most general liver disease characterized by a continuum of liver abnormalities ranging from simple fatty liver to advanced stage of nonalcoholic steatohepatitis, cirrhosis, and even hepatocellular carcinoma. The pathological drivers of NAFLD are complex and largely undefined. It is increasingly identified that the imbalance between renin-angiotensin system and ACE2/Ang-(1-7)/Mas axis, as well as mitochondrial dysfunction associated with NAFLD. However, no known empirical research has focused on exploring the effect of the regulation of mitochondrial respiration chain activity by Ang-(1-7)/Mas on the prevention of NAFLD. Here, we evaluated the interaction and relevance of hepatic Ang-(1-7)/Mas-axis challenge with glucolipid metabolism and mitochondrial condition in vivo and in vitro. In this context, we found that Mas deletion in mice contributed to the severe glucose intolerance, insulin resistance, and hepatic steatosis which accompanied by elevated levels of serum/ hepatic alanine aminotransferase, aspartate aminotransferase, and triglycerides, as well as the mitochondrial dysfunction. Whereas forced upregulation of Mas or Ang-(1-7) administration could significantly attenuate these consequences by downregulating the expression of hepatic lipogenic proteins and enzymes for gluconeogenesis. Furthermore, activation of Ang-(1-7)/Mas arm could improve the IRS-1/Akt/AMPK pathway and enhance the mitochondrial energy utilization. Considered together, it is becoming extremely hopeful to provide a new perspective for Ang-(1-7)/Mas axis for the therapeutics of NAFLD.