An aerotaxis transducer gene from Pseudomonas putida

Abstract An aerotaxis gene, aer, was cloned from Pseudomonas putida PRS2000. A P. putida aer mutant displayed an altered aerotactic response in a capillary assay. Wild-type P. putida clustered at the air/liquid interface. In contrast, the aer mutant did not cluster at the interface, but instead form...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 182; no. 1; pp. 177 - 183
Main Authors Nichols, Nancy N., Harwood, Caroline S.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.01.2000
Blackwell
Oxford University Press
Subjects
Aerotaxis
Bacteriology
Behavioral response
Biological and medical sciences
Carrier Proteins - chemistry
Carrier Proteins - genetics
Chemotaxis - genetics
Cloning, Molecular
Culture Media
E coli
Energy Metabolism - physiology
Energy taxis
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetics
Kanamycin Resistance - genetics
Microbiology
Molecular Sequence Data
Mutagenesis, Insertional
PAS domain
Proteins
Pseudomonas
Pseudomonas putida
Pseudomonas putida - genetics
Pseudomonas putida - physiology
Regulatory proteins
Signal Transduction - genetics
Succinic Acid - metabolism
Taxis
Transducer
Transducers
Transducer
PAS domain
Aerotaxis
Behavioral response
Energy taxis
Pseudomonadales
Gene
Taxis
Bacteria
Pseudomonadaceae
Molecular cloning
Pseudomonas putida
Anemotaxis
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Summary:Abstract An aerotaxis gene, aer, was cloned from Pseudomonas putida PRS2000. A P. putida aer mutant displayed an altered aerotactic response in a capillary assay. Wild-type P. putida clustered at the air/liquid interface. In contrast, the aer mutant did not cluster at the interface, but instead formed a diffuse band at a distance from the meniscus. Wild-type aer, provided in trans, complemented the aer mutant to an aerotactic response that was stronger than wild-type. The P. putida Aer sequence is similar over its entire length to the aerotaxis (energy taxis) signal transducer protein, Aer, of Escherichia coli. The amino-terminus is similar to redox-sensing regulatory proteins, and the carboxy-terminus contains the highly conserved domain present in chemotactic transducers.
Bibliography:Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb08893.x