Kainic acid and 1'-hydroxykainic acid from palmariales

The distribution of kainic acid among various red algae was investigated. Analysis of free amino acids from different populations of Palmaria palmata showed that some were unable to accumulate kainic acid to detectable concentrations, whereas in two dwarf mutants it was a major component of the free...

Full description

Saved in:
Bibliographic Details
Published inNatural toxins Vol. 2; no. 5; p. 286
Main Authors Ramsey, U.P. (Institute for Marine Biosciences, Halifax, Nova Scotia, Canada.), Bird, C.J, Shacklock, P.F, Laycock, M.V, Wright, J.L.C
Format Journal Article
LanguageEnglish
Published United States 1994
Subjects
ACIDE AMINE
Amino Acids - analysis
AMINOACIDOS
Chemical Fractionation
Chromatography, Ion Exchange
Culture Media
Kainic Acid - analogs & derivatives
Kainic Acid - chemistry
Kainic Acid - isolation & purification
Kainic Acid - metabolism
Magnetic Resonance Spectroscopy
Mass Spectrometry
METABOLISME
METABOLISMO
Mutation - genetics
NITRATE
Nitrates - chemistry
NITRATOS
RHODOPHYCEAE
Rhodophyta - metabolism
SISTEMA NERVIOSO
SYSTEME NERVEUX
TEMPERATURA
TEMPERATURE
TOXINAS
TOXINE
Online AccessGet more information

Cover

More Information
Summary:The distribution of kainic acid among various red algae was investigated. Analysis of free amino acids from different populations of Palmaria palmata showed that some were unable to accumulate kainic acid to detectable concentrations, whereas in two dwarf mutants it was a major component of the free amino acid composition. The amino acid profiles were also examined for unknown amino acids in the search for possible intermediates in kainic acid biosynthesis. The only unknown amino acid present in P. palmata extracts was isolated and identified by NMR spectroscopy as 1'-hydroxykainic acid. This compound was found in all samples that contained kainic acid. To investigate the effect of growth conditions on kainic acid production different strains of P. palmata were grown at 5, 10, and 15 degrees C with or without added nitrate. No effect on production was observed, suggesting that the growth conditions in these experiments do not affect the level of gene expression in the pathway of kainic acid biosynthesis. Furthermore, changing the growth conditions did not induce synthesis of kainic acid in the non-producing strains of Palmariales
Bibliography:9561164
H60
ISSN:1056-9014
1522-7189
DOI:10.1002/nt.2620020507