The Effects of Propofol on Local Field Potential Spectra, Action Potential Firing Rate, and Their Temporal Relationship in Humans and Felines

• Published in: Frontiers in human neuroscience Vol. 7; p. 136
• Main Authors: Hanrahan, Sara J., Greger, Bradley, Parker, Rebecca A., Ogura, Takahiro, Obara, Shinju, Egan, Talmage D., House, Paul A.
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Research Foundation 2013; Frontiers Media S.A
• Subjects: Action potential; Anesthesia; Anesthesiology; Consciousness; Electrodes; Electroencephalography; Electrophysiological recording; Firing pattern; Firing rate; Human subjects; Information processing; Intravenous administration; local field potential; microelectrode array; Neocortex; Neuroscience; power spectrum; Propofol; γ-Aminobutyric acid A receptors; United States > US; Japan; power spectrum; local field potential; microelectrode array; consciousness; propofol
• ISSN: 1662-5161; 1662-5161
• DOI: 10.3389/fnhum.2013.00136

Propofol is an intravenous sedative hypnotic, which, acting as a GABAA agonist, results in neocortical inhibition. While propofol has been well studied at the molecular and clinical level, less is known about the effects of propofol at the level of individual neurons and local neocortical networks. We used Utah Electrode Arrays (UEAs) to investigate the effects of propofol anesthesia on action potentials (APs) and local field potentials (LFPs). UEAs were implanted into the neocortex of two humans and three felines. The two human patients and one feline received propofol by bolus injection, while the other two felines received target-controlled infusions. We examined the changes in LFP power spectra and AP firing at different levels of anesthesia. Increased propofol concentration correlated with decreased high-frequency power in LFP spectra and decreased AP firing rates, and the generation of large-amplitude spike-like LFP activity; however, the temporal relationship between APs and LFPs remained relatively consistent at all levels of propofol. The probability that an AP would fire at this local minimum of the LFP increased with propofol administration. The propofol-induced suppression of neocortical network activity allowed LFPs to be dominated by low-frequency spike-like activity, and correlated with sedation and unconsciousness. As the low-frequency spike-like activity increased and the AP-LFP relationship became more predictable firing rate encoding capacity is impaired. This suggests a mechanism for decreased information processing in the neocortex that accounts for propofol-induced unconsciousness.