Indirect Electrochemical Sensing of Radicals and Radical Scavengers in Biological Matrices

• Published in: Angewandte Chemie (International ed.) Vol. 46; no. 42; pp. 8079 - 8081
• Main Authors: Scholz, Fritz, López de Lara González, Gabriela, Machado de Carvalho, Leandro, Hilgemann, Maurício, Brainina, Khenia Z, Kahlert, Heike, Jack, Robert Smail, Minh, Dang Truong
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.01.2007; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: Adsorption; analytical methods; antioxidants; Dose-Response Relationship, Drug; Electrochemistry; Electrodes; Free Radical Scavengers - analysis; Free Radicals - analysis; Gold - chemistry; Humans; Hydrogen Peroxide - chemistry; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Lipopolysaccharides - pharmacology; Membranes, Artificial; Mercury - chemistry; Oxidation-Reduction; radicals; Reactive Oxygen Species - metabolism; ruthenium; Salmonella typhimurium - chemistry; Silver - chemistry; Silver Compounds - chemistry; Sulfhydryl Compounds - chemistry; Surface Properties
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.200702690

Breaking cover: Hydroxyl radicals OH. destroy the self‐assembled monolayer (SAM) of hexanethiol on gold and mercury electrodes and thus allow the electrochemical response of the redox couple Ru2+/3+ to be measured. On a completely SAM‐covered electrode the Ru2+/3+ signal is absent, thus the recovery of the signal following radical attack is a reliable measure of the radical activity.