Protective effects of insulin-like growth factor-I on the decrease in myogenic differentiation by ionizing radiation

• Published in: International journal of radiation biology Vol. 85; no. 2; pp. 153 - 158
• Main Authors: Sakurai, Tomonori, Ueda, Takanori, Kawai, Miyuki, Tobita, Hideki, Miyakoshi, Junji
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.01.2009; Taylor & Francis
• Subjects: Animals; C2C12; Cell Differentiation - drug effects; Cell Differentiation - radiation effects; Cell Line; immunofluorescence; Insulin-Like Growth Factor I - pharmacology; Mice; Muscle Fibers, Skeletal - cytology; Muscle Fibers, Skeletal - drug effects; Muscle Fibers, Skeletal - radiation effects; MyoD Protein - genetics; myotube formation; Radiation-Protective Agents - pharmacology; RNA, Messenger - analysis; Space life sciences; X-Rays
• ISSN: 0955-3002; 1362-3095
• DOI: 10.1080/09553000802641177

Purpose: The aim of the work is to evaluate the effects of insulin-like growth factor-1 (IGF-1) on the decrease in myotube formation induced by ionizing radiation. Materials and methods: We induced C2C12 cells to a myogenic linage following X-ray irradiation at 2 and 4 Gy. Myogenic differentiation was estimated using immnocytochemical staining with anti-myosin antibody, and the anti-myosin antibody positive areas, the total number of nuclei, the number of nuclei included in multinucleated myotubes per field, and the myotube formation ratio were analyzed. Results: In the myogenic differentiation in the presence of IGF-1, the decrease in anti-myosin antibody positive areas, the nuclei included in myotubes, and the myotube formation ratio induced by X-ray irradiation at 2 Gy was restored to control levels. Conclusions: The addition of IGF-1 protected against the decrease myotube formation induced by X-ray irradiation at 2 Gy. Since X-ray irradiation at 2 Gy is usually used for multi-fractionated irradiation in radiotherapy, our findings suggest that IGF-1 could be useful to protect against impairment of muscle repair induced by therapeutic dose radiation.