Genome-wide identification of PYL gene family in wheat: Evolution, expression and 3D structure analysis

• Published in: Genomics (San Diego, Calif.) Vol. 113; no. 2; pp. 854 - 866
• Main Authors: Lei, Pengzheng, Wei, Xiulan, Gao, Ruiting, Huo, Fulin, Nie, Xiaojun, Tong, Wei, Song, Weining
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2021
• Subjects: Conserved Sequence; Evolution, Molecular; Exons; Expression profile; Homology modeling; Introns; Multigene Family; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Protein Domains; PYL gene family; Triticum - genetics; Wheat; PYL gene family; Expression profile; Wheat; Homology modeling
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2020.12.017

Here, 38 wheat PYL genes (TaPYLs) belonging to 13 homoeologous groups were identified using the genome-search method, with 26 and 12 PYL genes identified in Triticum dicoccoides and Aegilops tauschii, respectively. Phylogenetic relationship, conserved domain and molecular evolution analysis revealed that PYL genes showed highly conservative between wheat and theprogenitors. Interaction network and miRNA target prediction found that TaPYLs could interact with the important components of ABA signaling pathway and Tae-miR966b-3p might be a hub regulator mediating wheat ABA signal network. Furthermore, the tissue-specific and stress-responsive TaPYLs were detected through RNA-seq analysis. Expressions of 10 TaPYLs were validated by QPCR analysis and the homoeologous genes showed significantly differential expression, suggesting subfunctionalization of them has occurred. Finally, 3D structures of the TaPYL proteins were predicted by homology modeling. This study lays the foundation for further functional study of PYL genes for development and stress tolerance improvement in wheat and beyond. •The PYL gene family was identified in wheat and its two progenitors at the genome-wide level.•PYL genes showed highly conservative in wheat and its progenitors from the perspective of sequence composition, exon-intron structure, conserved domain and 3D structure.•Expression profiles of these wheat PYL genes were systematically investigated using the available 58 RNA-seq samples.•Subfunctionalization has occurred in A, B and D homoeologous copies of wheat PYL genes .