Possible biological processes controlled by the circatidal clock in the mangrove cricket inferred from transcriptome analysis

Intertidal dwellers exhibit a circatidal rhythm corresponding to the tidal cycles, but its molecular mechanism remains largely unknown. The mangrove cricket Apteronemobius asahinai simultaneously exhibits circatidal and circadian rhythms in its locomotor activity. In A. asahinai, it is considered th...

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Published inBiological rhythm research Vol. 53; no. 6; pp. 908 - 920
Main Authors Takekata, Hiroki, Tachibana, Shin-Ichiro, Motooka, Daisuke, Nakamura, Shota, Goto, Shin G.
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 03.06.2022
Taylor & Francis Ltd
Subjects
Apteronemobius asahinai
Circadian rhythm
Circadian rhythms
circatidal rhythm
Crustaceans
Epicuticle
Gene disruption
Light effects
Locomotor activity
Melanization
RNA-Seq
Sclerotization
Tides
transcriptome analysis
Transcriptomes
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Summary:Intertidal dwellers exhibit a circatidal rhythm corresponding to the tidal cycles, but its molecular mechanism remains largely unknown. The mangrove cricket Apteronemobius asahinai simultaneously exhibits circatidal and circadian rhythms in its locomotor activity. In A. asahinai, it is considered that the circatidal rhythm is generated by the circatidal clock, and its molecular mechanism appears to differ from that of the circadian clock. For the molecular dissection, it is necessary to investigate the effects of a candidate gene disruption in several circatidal rhythms. However, the circatidal rhythms other than locomotor activity have not been examined in this species. Here, to identify biological processes related to the circatidal rhythm, we performed whole-transcriptome analysis using RNA sequencing with RNA samples extracted from whole crickets. Under a light-dark cycle without tidal stimuli, more contigs showed a diel expression pattern than a tidal expression pattern. Gene ontology (GO) enrichment analysis revealed that most GO terms overrepresented in contigs related to the tidal cycle were also overrepresented in contigs related to the diel cycle. GO terms specific to tide-related contigs were related to cuticle development, melanization, and sclerotization. These results suggest that physiological events in the cuticle may be controlled by the circatidal clock.
ISSN:0929-1016
1744-4179
DOI:10.1080/09291016.2020.1838747