A simple and novel method for the production of polyethylene terephthalate containing agar plates for the growth and detection of bacteria able to hydrolyze this plastic

Polyethylene terephthalate is used in the manufacture of many products. Microbes able to hydrolyze the plastic are known, and offer promise for practical waste management. Screening for hydrolysis usually involves unwieldy culturing in the presence of film. A rapid screening method based on culturin...

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Bibliographic Details
Published inJournal of microbiological methods Vol. 185; p. 106222
Main Author Charnock, Colin
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.06.2021
Subjects
Agar
Agar plate clear zone method
Bacteria
Bacteria - growth & development
Bacteria - isolation & purification
Bacteriological Techniques - methods
Biodegradation
Biodegradation, Environmental
Burkholderiales
Culture Media - chemistry
Hydrolysis
Plastics - metabolism
Polyethylene terephthalate
Polyethylene Terephthalates
Biodegradation
Polyethylene terephthalate
Bacteria
Agar plate clear zone method
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Summary:Polyethylene terephthalate is used in the manufacture of many products. Microbes able to hydrolyze the plastic are known, and offer promise for practical waste management. Screening for hydrolysis usually involves unwieldy culturing in the presence of film. A rapid screening method based on culturing on agar plates is here described. •Polyethylene terephthalate degrading microbes might aid recycling efforts.•Few microbes degrading polyethylene terephthalate have so far been found.•An agar-based test for detecting bacterial polyethylene terephthalate biodegradation is described.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2021.106222