Ultrafiltration for genipin recovery technologies after ultrasonic treatment of genipap fruit

Centrifugal ultrafiltration of genipap fruit extracts after mechanical/sonic enzymatic treatment was performed through 50, 30, 10 and 3kDa molecular weight cutoff (MWCO) (15min; 10°C; 9425g). Volume, volume retention factor (VRF), flux, genipin and glucose concentrations were determined. Separation...

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Published inBiocatalysis and agricultural biotechnology Vol. 4; no. 1; pp. 11 - 16
Main Authors Ramos-de-la-Peña, Ana Mayela, Renard, Catherine M.G.C., Montañez, Julio C., Reyes-Vega, María de la Luz, Contreras-Esquivel, Juan Carlos
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.01.2015
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Summary:Centrifugal ultrafiltration of genipap fruit extracts after mechanical/sonic enzymatic treatment was performed through 50, 30, 10 and 3kDa molecular weight cutoff (MWCO) (15min; 10°C; 9425g). Volume, volume retention factor (VRF), flux, genipin and glucose concentrations were determined. Separation efficiency, retention, transmission coefficients (RC, TC), and membrane selectivity were estimated. Reduction in MWCO increased volume in the retentate (7.6±0.0mL), decreased VRF (1.9±0.0) and flux (0.06±0.00mLcm−2min−1) after 3kDa ultrafiltration. Genipin and glucose reached equilibrium in retentate and permeate (17±3.3; 16±1.5mgmL−1; 41±2.2 and 41±4.8mgmL−1) after 3kDa ultrafiltration. The highest removal of genipin was 0.5±0.1. RC and TC were 0.5±0.1; selectivity for genipin was 0.9±0.0 after 3kDa separation.
ISSN:1878-8181
1878-8181
DOI:10.1016/j.bcab.2014.09.009