Megf10-related engulfment of excitatory postsynapses by astrocytes following severe brain injury

• Published in: CNS neuroscience & therapeutics Vol. 29; no. 10; pp. 2873 - 2883
• Main Authors: Zhuang, Yuan, Xu, Xiaojian, Li, Hao, Niu, Fei, Yang, Mengshi, Ge, Qianqian, Lu, Shenghua, Deng, Yu, Wu, Hongbin, Zhang, Bin, Liu, Baiyun
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.10.2023; John Wiley and Sons Inc
• Subjects: Animal cognition; Astrocytes; Cognitive ability; Ethanol; Glial fibrillary acidic protein; Hippocampus; Immunofluorescence; Laboratory animals; Life sciences; Lysosomes; Original; Phagocytes; Phagocytosis; Postsynaptic density proteins; Transcriptomes; Traumatic brain injury; traumatic brain injury; phagocytosis; Megf10; astrocytes; hippocampus
• ISSN: 1755-5930; 1755-5949
• DOI: 10.1111/cns.14223

To investigate astrocyte-related phagocytosis of synapses in the ipsilateral hippocampus after traumatic brain injury (TBI). We performed controlled cortical impact to simulate TBI in mice. Seven days postinjury, we performed cognitive tests, synapse quantification, and examination of astrocytic phagocytosis in association with Megf10 expression. During the subacute stage post-TBI, we found a reduction in excitatory postsynaptic materials in the ipsilateral hippocampus, which was consistent with poor performance in the cognitive test. The transcriptome data suggested that robust phagocytosis was responsible for this process. Coincidently, we identified phagocytic astrocytes containing secondary lysosomes that were wrapped around the synapses in the ipsilateral hippocampus. Moreover, a significant increase in the co-location of GFAP and PSD-95 in the CA1 region suggested astrocytic engulfment of excitatory postsynaptic proteins. After examining the reported phagocytic pathways, we found that both the transcription level and protein expression of Megf10 were elevated. Co-immunofluorescence of GFAP and Megf10 demonstrated that the expression of Megf10 was spatially upregulated in astrocytes, exclusively in the CA1 region, and was related to the astrocytic engulfment of PSD-95. Our study elaborated that the Megf10-related astrocytic engulfment of PSD-95 in the CA1 region of the ipsilateral hippocampus aggravated cognitive dysfunction following severe TBI.