Analysis of genetic diversity among selected populations of Phragmites australis world-wide

• Published in: Aquatic botany Vol. 64; no. 3; pp. 209 - 221
• Main Author: Koppitz, Heike
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.1999
• Subjects: Clonal distribution; Freshwater; Genetic similarity; PCR-fingerprinting; Phragmites australis; Random amplified polymorphic DNA (RAPD); Reed population; Europe; Random amplified polymorphic DNA (RAPD); Reed population; Clonal distribution; Phragmites australis; PCR-fingerprinting; Genetic similarity
• ISSN: 0304-3770; 1879-1522
• DOI: 10.1016/S0304-3770(99)00051-0

Genetic diversity among reed ( Phragmites australis) populations from 70 sites in Europe and from 12 sites in other continents was determined using the random amplified polymorphic DNA (RAPD) technique. Based on results from about 1000 reed samples screened with different primers, both polyclonal reed stands, where different clones co-exist either next to each other or intermingle, and monoclonal reed stands were found. Generally a higher diversity of clones was detected from sites on or near shore than in the water. Cluster analysis of genetic similarity coefficients from 30 to 80% demonstrated a high degree of genetic diversity among reed stands world-wide. Lower genetic diversity was detected among samples from any location, with coefficients ranging from 70 to 100%. In general, the genetic distances of investigated reed samples within Europe increase with increasing geographic distances. The reed samples from the other continents formed a separate major cluster in the phenogram.