A novel bilateral grafting technique for studying patterning in Hydra

• Published in: Developmental biology Vol. 462; no. 1; pp. 60 - 65
• Main Authors: Wang, Rui, Collins, Eva-Maria S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2020
• Subjects: Acyclic Monoterpenes - pharmacology; Animals; Body Patterning - genetics; Cell displacement; Chimera - genetics; Grafting; Hydra; Hydra - genetics; Hydra - metabolism; Linalool; Patterning; Regeneration - genetics; Tissue Transplantation - methods; Transplants - physiology; Patterning; Hydra; Grafting; Linalool; Cell displacement
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/j.ydbio.2020.03.006

Control of patterning and the specification of body axes are fundamental aspects of animal development involving complex interactions between chemical, physical, and genetic signals. The freshwater polyp Hydra has long been recognized as a useful model system to address these questions due to its simple anatomy, optical transparency, and strong regenerative abilities, which enabled clever grafting experiments to alter and probe patterning. Reliable methods exist for the transplantation of small tissue pieces into the body column or the combination of sections cut perpendicular to the body axis, which can be used to examine oral-aboral gradients and axis induction potential of tissue fragments. However, existing methods do not allow researchers to probe questions of axis alignment and lateral information exchange. We therefore developed a technique to produce chimeric animals split longitudinally along the body axis of the animal by anesthetizing the animals with the terpene linalool and threading the donor pieces onto pairs of fine glass needles. Our novel approach can be applied to study questions in Hydra research that have thus far been inaccessible, including patterning processes acting perpendicular to the oral-aboral axis and the extent of lateral cell migration. •New method for creating chimeric Hydra split parallel to the head/foot axis.•Experimental confirmation that epithelial cells are not displaced circumferentially.•Alsterpaullone treatment and bilateral grafts can be combined to alter axial patterning.