DNA Copy Number Variation and Gene Expression Analyses Reveal the Implication of Specific Oncogenes and Genes in GBM

• Published in: Cancer investigation Vol. 27; no. 5; pp. 541 - 548
• Main Authors: Margareto, Javier, Leis, Olatz, Larrarte, Eider, Pomposo, Iñigo C., Garibi, Jesús María, Lafuente, José Vicente
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.01.2009; Taylor & Francis
• Subjects: Aged; Biomarkers, Tumor - genetics; CGH array; Chromosome Aberrations; Chromosomes, Human - genetics; Comparative Genomic Hybridization; Copy number gains; DNA, Neoplasm - genetics; Female; Gene Dosage; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Glioblastoma; Glioblastoma - genetics; Humans; Male; Middle Aged; Oligonucleotide Array Sequence Analysis; Oncogenes
• ISSN: 0735-7907; 1532-4192
• DOI: 10.1080/07357900802563044

To understand the pathogenesis of glioblastoma multiforme (GBM) we used high-resolution comparative genomic hybridization arrays and gene expression microarrays to identify DNA copy number alterations and gene expression changes in comparable sets of GBM samples. Gains were detected at chromosomes 1, 2, 7, 9, 12, 19, and 20 and losses at 6, 9, and 10. Gene expression analyses identified specific genes overexpressed in GBM mapping at amplified chromosomal regions. Among these genes we found genes involved in angiogenesis, extracellular matrix remodeling and several oncogenes. DNA copy number analysis along with gene expression profiles provides a powerful strategy to understand tumor progression and identification of genes involved in GBM pathogenesis.