Spatially resolved proteomics in osteoarthritis: State of the art and new perspectives

• Published in: Journal of proteomics Vol. 215; p. 103637
• Main Authors: Eveque-Mourroux, M.R., Rocha, B., Barré, F.P.Y., Heeren, R.M.A., Cillero-Pastor, B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 20.03.2020
• Subjects: Molecular imaging; Osteoarthritis; Proteomics; Spatially resolved; Molecular imaging; Spatially resolved; Osteoarthritis; Proteomics
• ISSN: 1874-3919; 1876-7737
• DOI: 10.1016/j.jprot.2020.103637

Osteoarthritis (OA) is one of the most common diseases worldwide caused by chronic degeneration of the joints. Its high prevalence and the involvement of several tissues define OA as a highly heterogeneous disease. New biological markers to evaluate the progression of the pathology and improve its prognosis are needed. Among all the different -omic strategies applied to OA, solution phase bottom-up proteomics has made an extensive contribution to the field of biomarker research. However, new technologies for protein analysis should be considered for a better understanding of the disease. This review focuses on complementary proteomic methodologies and new technologies for translational research of OA and other rheumatic pathologies, especially mass spectrometry imaging and protein imaging methods not applied by the OA community yet. [Display omitted] •Spatially resolved proteomic approaches can reveal the molecular interplay among different tissues in the joint.•MSI-guided proteomics can overcome the existing challenges for protein identification.•Multimodal imaging approaches provide insight on the molecular mechanisms that lead to OA and other rheumatic diseases.