Comparative study of the enzymatic synthesis of cephalexin at high substrate concentration in aqueous and organic media using statistical model

• Published in: Biotechnology and bioprocess engineering Vol. 17; no. 4; pp. 711 - 721
• Main Authors: Bahamondes, Carola, Pontificia Universidad Catolica de Valparaiso, Valparaiso, Chile, Wilson, Lorena, Pontificia Universidad Catolica de Valparaiso, Valparaiso, Chile, Aguirre, Carolina, Universidad Catolica de la Ssma. Concepcion Avenida Brasil 2147, Valparaiso, Chile, Illanes, Andres, Pontificia Universidad Catolica de Valparaiso, Valparaiso, Chile
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society for Biotechnology and Bioengineering 01.08.2012; Springer Nature B.V; 한국생물공학회
• Subjects: Analysis; Antibiotics; beta-lactam antibiotics; Biocatalysts; Biotechnology; Cephalexin; Chemical synthesis; Chemistry; Chemistry and Materials Science; Comparative studies; ENZIMAS INMOVILIZADAS; Enzymatic synthesis; ENZYME IMMOBILISEE; Enzymes; Ethylene glycol; Green chemistry; Hydrolysis; IMMOBILIZED ENZYMES; Industrial and Production Engineering; Mathematical models; Nucleophiles; Objective function; Penicillin; penicillin acylase; Penicillin amidase; pH effects; phenylglycine methyl ester; Productivity; Research Paper; Solvents; Statistical analysis; Statistical models; Studies; Substrates; Temperature effects; 생물공학; β-lactam antibiotics; immobilized enzyme; cephalexin; penicillin acylase
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-011-0674-6

Synthesis of cephalexin with immobilized penicillin acylase at high substrates concentration at an acyl donor to nucleophile molar ratio of 3 was comparatively evaluated in aqueous and ethylene glycol media using a statistical model. Variables under study were temperature, pH and enzyme to substrate ratio and their effects were evaluated on cephalexin yield, ratio of initial rates of cephalexin synthesis to phenylglycine methyl ester hydrolysis, volumetric and specific productivity of cephalexin synthesis, that were used as response parameters. Results obtained in both reaction media were modeled using surface of response methodology and optimal operation conditions were determined in terms of an objective function based on the above parameters. At very high substrates concentrations the use of organic co-solvents was not required to attain high yields and actually almost stoichiometric yields were obtained in a fully aqueous media with the advantages of higher productivities than in an organic co-solvent media and compliance with the principles of green chemistry.