Responses of lipid metabolism and lipidomics in the hepatopancreas of Pacific white shrimp Litopenaeus vannamei to microcystin-LR exposure

• Published in: The Science of the total environment Vol. 820; p. 153245
• Main Authors: Duan, Yafei, Zeng, Shimin, Lu, Zijun, Dan, Xueming, Mo, Zequan, Xing, Yifu, Zhang, Jiasong, Li, Yanwei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.05.2022
• Subjects: Animals; autophagy; carboxylic ester hydrolases; ceramides; environment; fatty acid metabolism; Gene expression; Hepatopancreas; homeostasis; isozymes; Lipid Metabolism; Lipidomics; lipogenesis; lipolysis; Litopenaeus vannamei; long chain fatty acids; Marine Toxins; microcystin-LR; Microcystins; Microcystins - metabolism; phosphatidylcholines; Shrimp; sphingomyelins; toxicity; triacylglycerols; Lipidomics; Lipid metabolism; Gene expression; Shrimp; Microcystins
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.153245

Microcystin-LR (MC-LR) is a toxic substance that threatens the health of aquatic animals. Hepatopancreas is the target organ of MC-LR toxicity. In this study, we investigated the effects of MC-LR on hepatopancreas lipid metabolism and lipidomic responses in Litopenaeus vannamei. After MC-LR exposure for 72 h, the hepatopancreas showed obvious tissue damage, and the activities of several lipase isoenzymes were decreased. Furthermore, the relative gene expression levels of lipolysis (CPT1, AMPKα), lipogenesis (SREBP, FAS, ACC, 6PGD), and long-chain fatty acid β-oxidation (ACDL, ACDVL, ACBP) were increased. MC-LR exposure also affected lipidomics homeostasis. Specifically, the levels of glycerophospholipids (phosphatidylcholine, phosphatidic acid, lyso-phosphatidylcholine, lyso-phosphatidylethanolamine, lyso-phosphatidylglycerol), sphingolipids (sphingomyelin and ceramides) and cholesteryl ester were increased, and those of phosphatidylinositol and triglyceride were decreased. The significantly altered lipid molecules were mainly associated with the pathways of lipid and fatty acid metabolism and autophagy. These results reveal that MC-LR exposure influences lipid metabolism and lipidomic homeostasis in the shrimp hepatopancreas. [Display omitted] •Hepatopancreas showed histological damage and decreased activities of lipases.•Higher gene expression of enzymes for lipolysis, lipogenesis and long-chain fatty acids β-oxidation.•Alteration of lipidomics homeostasis was associated with fatty acid metabolism and autophagy.•Lipid markers for glycerophospholipids, sphingolipids, cholesteryl ester and triacylglycerides were identified.