Selection of cowpea genotypes based on grain mineral and total protein content

• Published in: Acta agriculturae Scandinavica. Section B, Soil and plant science Vol. 69; no. 2; pp. 155 - 166
• Main Authors: Gerrano, Abe Shegro, Jansen van Rensburg, Willem S., Venter, Sonja L., Shargie, Nemera G., Amelework, Beyene A., Shimelis, Hussein A., Labuschagne, Maryke T.
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 17.02.2019; Taylor & Francis LLC; Taylor & Francis Group
• Subjects: Atomic absorption analysis; Bi-plot; Breeding; Calcium; cluster analysis; Composition; Copper; cowpea; Cowpeas; Cultivars; Dietary minerals; genetic diversity; Genetic resources; Genotypes; Grain; Human nutrition; Iron; Legumes; Magnesium; Manganese; mineral; Minerals; Nutrient content; Nutrients; Nutrition; Parents; Phosphorus; Population genetics; Potassium; principal component; Principal components analysis; protein; Protein composition; Proteins; Sodium; Spectral analysis; Spectrophotometry; Variance analysis; Vigna unguiculata; Zinc
• ISSN: 0906-4710; 1651-1913
• DOI: 10.1080/09064710.2018.1520290

Grain legumes, including cowpea, are the cheapest sources of minerals and protein to enhance human nutrition. Cultivar development and deployment of cowpea with increased grain mineral content and protein composition rely on selection of genetically unique and complementary breeding lines. The objective of this study was to assess the grain minerals and protein composition of diverse cowpea collections of eastern, southern and western Africa to select promising parents to develop a breeding population. Twenty-two genetically diverse cowpea genotypes were field evaluated using a randomised complete block design with three replications in two locations in South Africa. The dried grain mineral contents were determined using an Atomic Absorption Spectrophotometre, while the protein content was determined by the combustion method. Analyses of variance showed significant (P < 0.05) effects of genotypes, locations and their interactions. The test genotypes showed considerable variation for the following nine mineral contents: calcium (Ca), copper (Cu), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), sodium (Na), phosphorus (P), and zinc (Zn) varying from 0.07 to 0.12 mg kg −1 ; 4.46 to 7.81 mg kg −1 ; 0.06 to 0.11 g; 11.82 to 14.45 g; 1.86 to 2.27 g; 9.24 to 13.30 mg kg −1 ; 0.26 to 0.80 g; 4.63 to 5.92 g and 0.03.00 to 0.05 g, respectively. Further, the total protein content varied from 23.16 to 28.13%. Significant correlations were detected among some mineral elements and total protein content, suggesting the possibility of simultaneous selection for these traits. The principal component analysis (PCA) identified four principal components (PCs) contributing to 70.93% of the total explained variation amongst genotypes. Overall the following genotypes with desirable grain mineral and protein attributes were selected: IT90K-59 (Ca), 98K-5301 (Ca and protein), ITOOK-1060 (Cu), ITOOK-1217 and IT845-2246 (Fe), Bensogla (K, Na, and P), TVU11424 and ITOOK-1217 (Mg), CH14 (Mn and Na), TVU12637 (Mn) and Glenda and Vuli (Zn). The selected cowpea genotypes are useful genetic resources for population and cultivar development for grain nutrients composition.