GhTULP34, a member of tubby-like proteins, interacts with GhSKP1A to negatively regulate plant osmotic stress

• Published in: Genomics (San Diego, Calif.) Vol. 113; no. 1; pp. 462 - 474
• Main Authors: Li, Zhanshuai, Wang, Xiaoyan, Cao, Xiaocong, Chen, Baizhi, Ma, Changkai, Lv, Jiaoyan, Sun, Zhimao, Qiao, Kaikai, Zhu, Longfu, Zhang, Chaojun, Fan, Shuli, Ma, Qifeng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2021
• Subjects: Cotton; F-Box Proteins - genetics; F-Box Proteins - metabolism; GhTULP34; Gossypium - genetics; Gossypium - metabolism; Osmotic Pressure; Osmotic stress; Plant Proteins - genetics; Plant Proteins - metabolism; Protein Binding; Root length; Seed germination; SKP Cullin F-Box Protein Ligases - genetics; SKP Cullin F-Box Protein Ligases - metabolism; Stomatic movement; GhTULP34; Cotton; Stomatic movement; Root length; Seed germination; Osmotic stress
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2020.09.024

Tubby-like protein genes (TULPs), present in the form of large multigene families, play important roles in environmental stress. However, little is known regarding the TULP family genes in cotton. In this study, we systematically identified and analyzed the membership, characterization, and evolutionary relationship of TULPs in four species of cotton. Transcriptome analysis indicated that GhTULPs participate in environmental stress and cotton tissue development. At the same time, we also predicted and analyzed the potential molecular regulatory mechanisms and functions of TULPs. GhTULP34, as a candidate gene, significantly reduced the germination rate of transgenic Arabidopsis plants under salt stress, and inhibited root development and stomatal closure under mannitol stress. The yeast two-hybrid and luciferase (LUC) systems showed that GhTULP34 can interact with GhSKP1A, a subunit of the SCF-type (Skp1-Cullin-1-F-box) complex. This study will provide a basis and reference for future research on their roles in stress tolerance. •We identified 107 candidate TULP genes in four cotton species.•Overexpression of GhTULP34 decreases salt tolerance in transgenic Arabidopsis.•GhTULP34 interacts with GhSKP1A, the subunit of SCF-type complex, to form a functional SCF-type E3 ligase, and then participates in the response to negative regulation of osmotic stress during seed germination and seedling growth.