Counter-receptors on human basophils for endothelial cell adhesion molecules

• Published in: The Journal of immunology (1950) Vol. 157; no. 2; pp. 844 - 850
• Main Authors: Bochner, BS, Sterbinsky, SA, Briskin, M, Saini, SS, MacGlashan, DW, Jr
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15.07.1996
• Subjects: Animals; Antibodies, Monoclonal - chemistry; Basophils - metabolism; Binding, Competitive - immunology; Cell Adhesion Molecules - immunology; Cell Adhesion Molecules - metabolism; CHO Cells; Cricetinae; Endothelium, Vascular - metabolism; Humans; Immunoglobulins - metabolism; Intercellular Adhesion Molecule-1 - metabolism; Mucoproteins - metabolism; Receptors, Cell Surface - metabolism; Receptors, Lymphocyte Homing - metabolism; Vascular Cell Adhesion Molecule-1 - metabolism
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.157.2.844

Ligands on human basophils for the endothelial adhesion molecules intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), mucosal addressin cell adhesion molecule-1 (MAdCAM-1), and E-selectin were investigated. Adhesion of basophils to endothelial cells was inhibited by mAb recognizing CD18, CD11a, and/or CD11b, with the pattern and magnitude of inhibition dependent upon the activation state of the basophils and endothelium. Adhesion to recombinant VCAM-1 was completely inhibited by mAb recognizing alpha 4 integrin and partially by mAb to the beta 1 or beta 7 subunit; surface expression of these integrins was also detected. Adhesion to recombinant MAdCAM-1 expressed on Chinese hamster ovary cells was completely inhibited by mAb recognizing alpha 4 and/or beta 7 integrins. Adhesion to recombinant E-selectin was completely inhibited by basophil pretreatment with neuraminidase and partially inhibited by endo-beta-galactosidase. By flow cytometry, bimodal patterns of expression of sialyl-Lewis X- and sialyl-dimeric-Lewis X were observed, and adherent cells tended to be sialyl-dimeric-Lewis X positive. Thus, basophils express beta 1, beta 2, and beta 7 integrins along with sialylated surface ligands that may interact with the endothelium during basophil recruitment responses.