Spray-drying Microencapsulation of an Extract from Tilia tomentosa Moench Flowers: Physicochemical Characterization and in Vitro Intestinal Activity

• Published in: Plant foods for human nutrition (Dordrecht) Vol. 77; no. 3; pp. 467 - 473
• Main Authors: Mainente, Federica, Piovan, Anna, Zanoni, Francesca, Chignola, Roberto, Cerantola, Silvia, Faggin, Sofia, Giron, Maria Cecilia, Filippini, Raffaella, Seraglia, Roberta, Zoccatelli, Gianni
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2022; Springer Nature B.V
• Subjects: Animal models; Animals; Biological activity; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Contractility; Dietary supplements; Drying; Ecology; Encapsulation; Extreme values; Flavanols; Flavonols; Flowers; Flowers - chemistry; Food Science; Functional foods & nutraceuticals; Glycosides; Health promotion; Intestine; Kaempferol; Kaempferols - analysis; Liquid chromatography; Maltodextrin; Mice; Microencapsulation; Nutrition; Original Paper; Phenols; Plant Extracts - chemistry; Plant Physiology; Quercetin; Quercetin - analysis; Spectrophotometry; Stability; Starch; Starch - chemistry; Tilia - chemistry; Tilia tomentosa; Moench; Encapsulation; Flavonols; Intestinal contractile response; Octenyl succinic anhydride-modified starch; Phenolic compounds; Silver linden; Silver linden (Tilia tomentosa Moench)
• ISSN: 0921-9668; 1573-9104
• DOI: 10.1007/s11130-022-00995-y

Silver linden ( Tilia tomentosa Moench, TtM ) flowers possess several health-promoting properties, especially at the neurological level, such as intestinal relaxation activity associated with specific flavonols, particularly quercetin and kaempferol derivatives. However, such molecules are susceptible to degradation upon different triggers like heat, light and extreme pH values. To overcome the scarce stability of TtM flowers bioactive molecules and make them suitable for developing functional food and supplements, we applied microencapsulation. Spray-drying microencapsulation of TtM flowers extract was performed using three starch-derived wall materials: maltodextrin 12 DE (MD12) and 19 DE (MD19), and OSA-modified starch (OSA-S). The stability of total phenols, flavanols, and antioxidant capacity was monitored for 70 days under accelerated stress conditions (40 °C/70% RH) by HPLC and spectrophotometric methods, and the intestinal contractile activity was tested in a murine model. In comparison to MD12 and MD19, OSA-S stood out for the higher encapsulation efficiency of quercetin and kaempferol glycosides (+ 36–47% compared to MD12 and + 18–24% compared to MD19) and stability thereof (half-life on average + 30% compared to MD12 and + 51% compared to MD19). The intestinal contractile activity of OAS-S powders resulted comparable to the original extract, indicating that flavonols were biologically active and accessible. Our results underly the potential advantages of OSA-S encapsulated formulation as a functional ingredient for the development of nutraceutical products.