Effects of a fully enclosed hollow-fiber centrifugal ultrafiltration technique for laboratory biosafety improvement

Laboratory biosafety has become a core focus in biological analysis, owing to the frequent occurrence of laboratory-acquired infections caused by the leakage of pathogenic microorganisms. For this purpose, the authors developed a safe pretreatment device combining a sealing technique with a direct i...

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Published inBioTechniques Vol. 71; no. 3; pp. 465 - 472
Main Authors Yang, Jing, Wang, Weigang, Qiang, Cuixin, Niu, Yanan, Li, Zhirong, Zhao, Mengqiang, Jiang, Ye, Cao, Jing, Niu, Xiaoran, Liu, Xiaoxuan, Zhao, Jianhong
Format Journal Article
LanguageEnglish
Published England Future Science Ltd 01.09.2021
Taylor & Francis Group
Subjects
bacterium
centrifuge tube
Containment of Biohazards
fully enclosed hollow fiber centrifugal ultrafiltration
Hepacivirus
Hepatitis B virus
Laboratories
laboratory biosafety
laboratory-acquired infections
Ultrafiltration
virus
bacterium
laboratory biosafety
laboratory-acquired infections
virus
fully enclosed hollow fiber centrifugal ultrafiltration
centrifuge tube
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Summary:Laboratory biosafety has become a core focus in biological analysis, owing to the frequent occurrence of laboratory-acquired infections caused by the leakage of pathogenic microorganisms. For this purpose, the authors developed a safe pretreatment device combining a sealing technique with a direct injection technique. In this study, several bacteria and viruses were used to validate the filtration effect of the invention. Data show that the new device can completely filter bacteria and that the filtration rates for hepatitis B virus and hepatitis C virus reached 94% and 96%, respectively. The results show that the new preparation device can effectively block these pathogens and can improve biological safety and provide powerful protection for technicians. The study describes a sealed sample pretreatment device that prevents experimenters from being exposed to biofluids, that is, fully enclosed hollow-fiber centrifugal ultrafiltration (FE-HFCF-UF). FE-HFCF-UF can quickly and conveniently obtain high-quality ultrafiltrate samples for small molecule analysis, while effectively filtrating pathogens and playing an active defense role for experimenters.
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ISSN:0736-6205
1940-9818
DOI:10.2144/btn-2021-0029