One-step separation of terpenoids from leaves extracts of Solanum cernuum by high-performance countercurrent chromatography

• Published in: Journal of liquid chromatography & related technologies Vol. 39; no. 1; pp. 8 - 12
• Main Authors: Ramos, Amaro Chaves, de Oliveira, Rodrigo Rodrigues
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 02.01.2016; Taylor & Francis Ltd
• Subjects: Acetonitrile; Chemical composition; Chromatography; Countercurrent chromatography; gas chromatography; Hexanes; Methyl alcohol; Separation; solanaceae; Solanum; Solanum cernuum; solvent system; Solvents; Terpenes; terpenoids
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826076.2015.1115767

Solanum cernuum is a native shrub from Brazil that has been used as medicine since the nineteenth century. Although it has been utilized for a long time, there are few studies regarding its chemical composition and it motivated us to investigate this species. The dried leaves of S. cernuum were extracted with methanol and the obtained extract was further partitioned with different organic solvents. As part of our investigation, the n-hexane fraction was submitted to CCC with a nonaqueous solvent system composed of hexane, acetonitrile, and ethyl acetate (5:5:1, v/v/v). Three terpenoids were obtained by one-step separation and identified as 24-oxo-31-norcycloartanone (22.8 mg), lupeol (9.4 mg), and cycloeucalenone (13.4 mg). Complementarily, the solvents system used on the separation have had their compositions determined by gas chromotography-flame ionization detector (GC-FID).