Gamma-hydroxybutyrate, acting through an anti-apoptotic mechanism, protects native and amyloid-precursor-protein-transfected neuroblastoma cells against oxidative stress-induced death

• Published in: Neuroscience Vol. 263; pp. 203 - 215
• Main Authors: Wendt, G, Kemmel, V, Patte-Mensah, C, Uring-Lambert, B, Eckert, A, Schmitt, M.J, Mensah-Nyagan, A.G
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 28.03.2014
• Subjects: Adult and adolescent clinical studies; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - metabolism; Apoptosis - drug effects; Biological and medical sciences; Cell Line, Tumor; Cell Survival - drug effects; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Humans; Medical sciences; Neuroblastoma; Neurology; Neuroprotective Agents - pharmacology; Organic mental disorders. Neuropsychology; Oxidative Stress; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; Sodium Oxybate - pharmacology; Transfection; GHB; NADPH; amyloid-precursor-protein; fluorescence-activated cell sorting; FACS; phosphate-buffered saline; MTT; BSA; DMEM; anti-apoptotic drug; FITC; TUNEL; OD; ethylenediaminetetraacetic acid; sodium dodecyl sulfate; qPCR; fluorescein isothiocyanate; Dubelcco’s modified eagle medium; PBS; AD; SDS; mechanisms of action of GHB; Alzheimer’s disease; APPwt; hydrogen peroxide; nicotinamide adenine dinucleotide phosphate; anti-oxidant drug; quantitative polymerase chain reaction; EDTA; 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium-bromide; H 2O 2; phycoerythrin; PE; neuroprotection; gamma-hydroxybutyrate; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; optical density; bovine serum albumin; Neuroprotection; Oxidative stress; Nervous system diseases; Alzheimer disease; Sodium oxybate; Amyloid precursor protein; Cerebral disorder; Cell line; Neuron; Cell death; Central nervous system disease; Degenerative disease; Alzheimer's disease; Apoptosis
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.12.067

Highlights • GHB reduces H2 O2 -induced native and APP-transfected SH-SY5Y cell death. • GHB decreases APP-overexpression or H2 O2 -evoked SH-SY5Y cell apoptosis. • GHB down-regulates H2 O2 or APP-overexpression-induced Bax/Bcl-2 mRNA ratio increase. • GHB does not promote native or APP-transfected SH-SY5Y cell proliferation. • We conclude that GHB exerts neuroprotective effects via an anti-apoptotic mechanism.