Hydrolysis of inositol phospholipids induced by stimulation of the T cell antigen receptor complex in antigen-specific, murine helper T cell clones. Requirement for exogenous calcium

• Published in: The Journal of immunology (1950) Vol. 143; no. 2; pp. 587 - 595
• Main Authors: Bonvini, E, DeBell, KE, Kolber, MA, Hoffman, T, Hodes, RJ, Taplits, MS
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15.07.1989
• Subjects: Animals; Antibodies, Monoclonal - physiology; Antigen-Presenting Cells - immunology; Antigen-Presenting Cells - metabolism; Calcium - pharmacology; Clone Cells - classification; Clone Cells - drug effects; Clone Cells - metabolism; Epitopes - immunology; Hemocyanins - immunology; Hydrolysis; Mice; Mice, Inbred C57BL; Microspheres; Phosphatidylinositols - immunology; Phosphatidylinositols - metabolism; Receptors, Antigen, T-Cell - drug effects; Receptors, Antigen, T-Cell - metabolism; T-Lymphocytes, Helper-Inducer - classification; T-Lymphocytes, Helper-Inducer - drug effects; T-Lymphocytes, Helper-Inducer - metabolism

Two murine, keyhole limpet hemocyanin-specific, Th cell clones were studied for their ability to respond to antibody-mediated stimulation of the TCR complex or to Ag-pulsed accessory cells by hydrolyzing inositol phospholipids. Both clones were positive for the determinant expressed on the epsilon c...