Use of Cotinine Immunoassay Test Strips for Preclassifying Urine Samples from Smokers and Nonsmokers Prior to Analysis by LC-MS-MS

Cotinine biomarker measurements involving both smokers and nonsmokers must accommodate a broad range of concentrations. Thus, we have routinely preclassified unknown samples as being either “high” or “low” by using an enzyme-linked immunoassay for cotinine prior to analysis by tandem mass spectromet...

Full description

Saved in:
Bibliographic Details
Published inJournal of analytical toxicology Vol. 29; no. 8; pp. 814 - 818
Main Authors Bernert, John T., Harmon, Tia L., Sosnoff, Connie S., McGuffey, James E.
Format Journal Article
LanguageEnglish
Published Niles, IL Oxford University Press 01.11.2005
Preston
Subjects
Biological and medical sciences
Medical sciences
Tobacco, tobacco smoking
Toxicology
Human
Urine
Biological fluid
Chemical analysis
CNS stimulant
Metabolite
Coupled method
Psychotropic
HPLC chromatography
Biological marker
Enzyme immunoassay
Electrospray
Immunological method
Non smoker
Smoker
Mass spectrometry MS/MS
Classification
Cotinine
Quantitative analysis
Nicotine
Online AccessGet full text

Cover

More Information
Summary:Cotinine biomarker measurements involving both smokers and nonsmokers must accommodate a broad range of concentrations. Thus, we have routinely preclassified unknown samples as being either “high” or “low” by using an enzyme-linked immunoassay for cotinine prior to analysis by tandem mass spectrometry (MS). Although this method is effective, it is also time-consuming and complex; a simpler and faster approach would be useful. Consequently, a screening assay for urine cotinine using an immunochromatographic test strip (NicAlert™) followed by a computerized analysis of the data was examined as a possible alternative. The results indicate that this approach can provide useful classification efficiency when using our target cutoff value of approximately 20 ng/mL. In the analysis of 50 urine samples from nonsmokers with varying degrees of exposure to environmental tobacco smoke, the classification sensitivity and specificity were 88% and 92%, respectively, for cotinine measured by the test strips relative to total cotinine concentrations measured by atmospheric-pressure ionization tandem MS. However, the relatively high cost of the strips may be a limiting factor.
Bibliography:ark:/67375/HXZ-1GFBDJDT-Q
Use of trade names is for identification only and does not constitute endorsement by the Public Health Service or the U.S. Department of Health and Human Services.
istex:F9F5B9197DAAD4932A81D069F59152ED62DFC3F8
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/29.8.814