Arboviruses and Blood Meal Sources in Zoophilic Mosquitoes at Human-Wildlife Interfaces in Kenya

• Published in: Vector borne and zoonotic diseases (Larchmont, N.Y.) Vol. 20; no. 6; p. 444
• Main Authors: Musa, Ali A, Muturi, Margaret W, Musyoki, Abednego M, Ouso, Daniel O, Oundo, Joseph W, Makhulu, Edward E, Wambua, Lillian, Villinger, Jandouwe, Jeneby, Maamun M
• Format: Journal Article
• Language: English
• Published: United States 01.06.2020
• Subjects: Animals; Animals, Wild - blood; Arboviruses - genetics; Arboviruses - isolation & purification; Culicidae - classification; Culicidae - physiology; Culicidae - virology; DNA - blood; DNA - genetics; Humans; Kenya; Livestock - blood; Mosquito Vectors; Phylogeny; Species Specificity; Kenya; mosquito blood meal; Aedes; Eretmapodites; dengue virus; zoophilic mosquitoes; Sindbis virus
• ISSN: 1557-7759
• DOI: 10.1089/vbz.2019.2563

Zoophilic mosquitoes play an important role in the transmission of arboviruses of medical importance at human-wildlife interfaces, yet arbovirus surveillance efforts have been focused mostly on anthropophilic mosquitoes. Understanding the diversity of zoophilic mosquitoes and their associated feeding patterns and arboviruses can inform better vector control strategies. We morphologically identified mosquitoes collected from two game reserves in Kenya, the Maasai Mara National Reserve (MMNR) and locations near the Shimba Hills National Reserve (SHNR). Representative mosquitoes were also identified by cytochrome c oxidase subunit 1 (COI) barcode sequencing. In addition, we identified the vertebrate hosts of mosquito blood meals from the contents of each mosquito's abdomen by high-resolution melting (HRM) analysis and sequencing of COI, 16S ribosomal RNA, and cytochrome b gene PCR products. Similarly, mosquito arbovirus infections were identified by HRM analysis and sequencing of and -specific RT-PCR products. Of 2858 mosquitoes collected, 51 were engorged with blood meals from seven different vertebrate hosts, including humans, birds, domestic, and peridomestic animals and wildlife. was the most abundant mosquito genus, with being the most abundant species in both study regions. Among MMNR samples, we detected dengue serotype-2 virus (DENV-2) for the first time in and , as well as Sindbis virus in male . We also detected DENV-2 in sampled from locations near the SHNR. Human and diverse wildlife blood meals were identified, including bushbuck blood in the dengue-infected and both human and hippopotamus blood in a single mosquito. Our findings highlight the potential risk of sylvatic dengue and Sindbis transmission to humans by zoophilic mosquitoes at human-wildlife interfaces in Africa. Of specific importance, we provide evidence of sylvatic DENV-2 in and representing potential new dengue vectors.