Co-translational formation of disulfides guides folding of the SARS-CoV-2 receptor binding domain

• Published in: Biophysical journal Vol. 122; no. 16; pp. 3238 - 3253
• Main Authors: Bitran, Amir, Park, Kibum, Serebryany, Eugene, Shakhnovich, Eugene I.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22.08.2023; The Biophysical Society
• Subjects: COVID-19; Disulfides - chemistry; Humans; Protein Folding; Proteins - chemistry; SARS-CoV-2
• ISSN: 0006-3495; 1542-0086; 1542-0086
• DOI: 10.1016/j.bpj.2023.07.002

Many secreted proteins, including viral proteins, contain multiple disulfide bonds. How disulfide formation is coupled to protein folding in the cell remains poorly understood at the molecular level. Here, we combine experiment and simulation to address this question as it pertains to the SARS-CoV-2 receptor binding domain (RBD). We show that the RBD can only refold reversibly if its native disulfides are present before folding. But in their absence, the RBD spontaneously misfolds into a nonnative, molten-globule-like state that is structurally incompatible with complete disulfide formation and that is highly prone to aggregation. Thus, the RBD native structure represents a metastable state on the protein’s energy landscape with reduced disulfides, indicating that nonequilibrium mechanisms are needed to ensure native disulfides form before folding. Our atomistic simulations suggest that this may be achieved via co-translational folding during RBD secretion into the endoplasmic reticulum. Namely, at intermediate translation lengths, native disulfide pairs are predicted to come together with high probability, and thus, under suitable kinetic conditions, this process may lock the protein into its native state and circumvent highly aggregation-prone nonnative intermediates. This detailed molecular picture of the RBD folding landscape may shed light on SARS-CoV-2 pathology and molecular constraints governing SARS-CoV-2 evolution.