In vitro induction of autotetraploid of Roman chamomile (Chamaemelum nobile L.) by colchicine treatment and essential oil productivity of its capitulum

• Published in: In vitro cellular & developmental biology. Plant Vol. 52; no. 5; pp. 479 - 483
• Main Authors: Tsuro, Masato, Kondo, Natsumi, Noda, Marii, Ota, Keiko, Nakao, Yoshinori, Asada, Satoshi
• Format: Journal Article
• Language: English
• Published: New York Springer Science + Business Media, LLC 01.10.2016; Springer US; Springer Nature B.V
• Subjects: Agricultural productivity; Biomass; Biomedical and Life Sciences; Cell Biology; Chamomile; Chromosomes; Developmental Biology; Diploidy; Essential oils; Flowers & plants; Industrial productivity; Life Sciences; Metabolites; Morphology; Oils & fats; Petroleum production; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant morphology; Plant Sciences; Plantlets; Productivity; Tetraploidy; Hairy Root; Chromosome Doubling; Tetraploid Plant; Tropane Alkaloid; Artemisinin
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-016-9779-0

In Artemisia annua, tetraploid hairy roots produced up to six times more sesquiterpene artemisinin than the diploid original (Jesus-Gonzalez and Weathers 2003). [...]enhanced productivities of essential oil as a secondary metabolite in tetraploid herbal plants have been reported, e.g., caraway (Dijkstra and Speckmann 1980) and Japanese peppermint (Janaki-Ammal and Sobti 1962). After 3 wk of culture, calli (about 7x7 mm) were transferred to 50 ml of liquid MS medium supplemented with the same plant growth regulators as in the callus induction with either 0,0.05, 0.1, and 0.5% (w/v) of colchicine (Wako Pure Chemical, Osaka, Japan) in 200 ml Erlenmeyer flask, cultured with horizontal gentle-rotation (50 rpm) at 25°C under dark conditions for 12 h. Then, they were washed three times with sterile distilled water and dried on sterile filter paper.