Nanocrystalline diamond film for biosensor applications

• Published in: Diamond and related materials Vol. 19; no. 5; pp. 457 - 461
• Main Authors: Qureshi, Anjum, Gurbuz, Yasar, Howell, Mick, Kang, Weng P., Davidson, Jimmy L.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2010; Elsevier
• Subjects: Antibody; Antigens; Biosensor; Biosensors; C-reactive protein; Capacitive; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Cross-disciplinary physics: materials science; rheology; Dielectric thin films; Dielectrics; Dielectrics, piezoelectrics, and ferroelectrics and their properties; Diseases; Electrodes; Exact sciences and technology; Fullerenes and related materials; diamonds, graphite; Materials science; Nanocrystalline diamond film; Nanocrystals; Nanoscale materials and structures: fabrication and characterization; Nanostructure; Other topics in nanoscale materials and structures; Physics; Proteins; Specific materials; C-reactive protein; Antibody; Capacitive; Biosensor; Nanocrystalline diamond film; Gold; Fourier transform spectroscopy; Synthetic diamond; Macromolecules; Antibodies; Immobilization; Contact angle; Polycrystalline diamond; Proteins; Dielectric thin films; Biosensors; Permittivity; Nanostructured materials
• ISSN: 0925-9635; 1879-0062
• DOI: 10.1016/j.diamond.2010.01.012

In this study, we have developed a novel capacitive biosensor based on interdigitated gold nanodiamond (GID-NCD) electrode for detection of C-reactive protein (CRP) antigen. CRP is one of the plasma proteins known as acute-phase proteins and its levels rise dramatically during inflammatory processes occurring in the body. It has been reported that CRP in serum can be used for risk assessment of cardiovascular diseases. The antibodies immobilization were confirmed by fourier transform spectroscopy (FTIR) and contact angle measurements. In this capacitive biosensor, nanocrystalline diamond acting as a dielectric layer between the electrodes. The CRP antigen detection was performed by capacitive/dielectric-constant measurements. Our results showed that the response of NCD-based capacitive-based biosensor for CRP antigen was dependent on both concentration (25–800 ng/ml) as well as frequency (50–350 MHz). Furthermore, using optimized conditions, the biosensors developed in this study can be potentially used for detection of elevated level of risk markers protein in suspected subjects for early diagnosis of disease.