Volumetric changes of the enteric nervous system under physiological and pathological conditions measured using x‐ray phase‐contrast tomography

• Published in: JGH open Vol. 8; no. 9; pp. e70027 - n/a
• Main Authors: Peruzzi, Niccolò, Eckermann, Marina, Frohn, Jasper, Salditt, Tim, Ohlsson, Bodil, Bech, Martin
• Format: Journal Article
• Language: English
• Published: Melbourne Wiley Publishing Asia Pty Ltd 01.09.2024; John Wiley & Sons, Inc; Wiley
• Subjects: Biopsy; Diabetes; dysmotility; enteric nervous system; histopathology; Hyperplasia; Large intestine; Medical records; Nervous system; Neurodegeneration; Neurons; Original; Small intestine; three‐dimensional; Tomography; volumetric measurements; X-rays; x‐ray phase‐contrast tomography; United States > US; histopathology; three‐dimensional; enteric nervous system; dysmotility; volumetric measurements; x‐ray phase‐contrast tomography
• ISSN: 2397-9070; 2397-9070
• DOI: 10.1002/jgh3.70027

Background and Aim Full‐thickness biopsies of the intestinal wall may be used to study and assess damage to the neurons of the enteric nervous system (ENS), that is, enteric neuropathy. The ENS is difficult to examine due to its localization deep in the intestinal wall and its organization with several connections in diverging directions. Histological sections used in clinical practice only visualize the sample in a two‐dimensional way. X‐ray phase‐contrast micro‐computed tomography (PC‐μCT) has shown potential to assess the cross‐sectional thickness and volume of the ENS in three dimensions (3D). The aim of this study was to explore the potential of PC‐μCT to evaluate its use to determine the size of the ENS. Methods Full‐thickness biopsies of ileum obtained during surgery from five controls and six patients clinically diagnosed with enteric neuropathy and dysmotility were included. Punch biopsies of 1 mm in diameter and 1 cm in length, from an area containing myenteric plexus, were extracted from paraffin blocks, and scanned with synchrotron‐based PC‐μCT without any staining. Results The microscopic volumetric structure of the neural tissue (consisting of both ganglia and fascicles) could be determined in all samples. The ratio of neural tissue volume/total tissue volume was higher in controls than in patients with enteric neuropathy (P = 0.013). The patient with the longest disease duration had the lowest ratio. Conclusion The assessment of neural tissue can be performed in an objective, standardized way, to ensure reproducibility and comparison under physiological and pathological conditions. Further evaluation is needed to examine the role of this method in the diagnosis of enteric neuropathy. The assessment of neural tissue in the enteric nervous system by X‐ray phase‐contrast micro‐computed tomography (PC‐µCT) showed that the ratio of neural tissue volume/total tissue volume was higher in controls than in patients with enteric neuropathy (P = 0.013). The patient with the longest disease duration had the lowest ratio. This ensures reproducibility and comparison under physiological and pathological conditions. Further evaluation is needed to examine the role of this method in the diagnosis of enteric neuropathy.