Development of Enzyme Biosensor Based on ISFETs for Quantitative Analysis of Serine Proteinases

• Published in: Electroanalysis (New York, N.Y.) Vol. 16; no. 22; pp. 1883 - 1889
• Main Authors: Biloivan, Olga A., Dzyadevych, Sergiy V., Boubriak, Olga A., Soldatkin, Alexey P., El'skaya, Anna V.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.11.2004; WILEY‐VCH Verlag
• Subjects: Ester artificial substrate of proteinase; pH-FET; Proteinase activity assay; Trypsin; α2-Macroglobulin
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/elan.200403076

A new potentiometric biosensor allowing quantitative determination of the proteinases through their esterase activity has been developed. The biosensor, specific for ester artificial substrates of serine proteinases has been fabricated using a pH‐sensitive field effect transistor (pH‐FET) and an immobilized complex of trypsin and α2‐macroglobulin. It has been shown that created biosensor is able to determine the activity of the soluble trypsin in the range of 0.1–30 U/mL (substrate BAEE). The relative standard deviation for the trypsin determination was approximately 3%. The operation stability of the biosensor was no less than 12 h (40 measurements). The response of the biosensor stored at +4 °C was stable for 30 days.