Secretomes of Gingival Fibroblasts From Periodontally Diseased Tissues: A Proteomic Analysis

• Published in: Clinical and experimental dental research Vol. 11; no. 1; pp. e70103 - n/a
• Main Authors: Smedås, Anne Kari, Paris, Lovise Gangeskar, Al‐Sharabi, Niyaz, Kristoffersen, Einar K., Sanz, Mariano, Mustafa, Kamal, Bunæs, Dagmar Fosså, Shanbhag, Siddharth
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.02.2025; John Wiley and Sons Inc; Wiley
• Subjects: Adult; Biopsy; Cell culture; Cells, Cultured; Chromatography, Liquid; conditioned media; Culture Media, Conditioned; Cytokines; Cytokines - metabolism; Dentistry; Extracellular vesicles; Female; Fibroblasts; Fibroblasts - metabolism; Gingiva - cytology; Gingiva - metabolism; Gingiva - pathology; Good Manufacturing Practice; Growth factors; Gum disease; Homeostasis; Humans; Hypothesis testing; in vitro; Male; mass spectrometry; Middle Aged; Original; Peptides; periodontal disease; periodontitis; Periodontitis - metabolism; Periodontitis - pathology; Proteins; Proteomics - methods; Secretome - metabolism; Software; Tandem Mass Spectrometry; Wound healing; United States > US; Norway; mass spectrometry; conditioned media; periodontal disease; in vitro; periodontitis
• ISSN: 2057-4347; 2057-4347
• DOI: 10.1002/cre2.70103

ABSTRACT Objective Cell secretomes represent a promising strategy for periodontal and bone regeneration. The objective of this study was to characterize the secretome of human gingival fibroblasts (GF) from periodontally diseased tissues (GF‐perio) using proteomics. Materials and Methods Conditioned media of GF‐perio from periodontitis patients (n = 6, 48‐h serum‐free culture) were subjected to liquid chromatography with tandem mass spectrometry. Global profiles, differentially expressed proteins (DEPs), and functional/gene‐set enrichment (FEA) were analyzed using bioinformatics. Selected bone regeneration‐related proteins were additionally measured using a multiplex immunoassay. Conditioned media of GF from periodontally healthy subjects were used as a reference. Results Overall, 1833 proteins were detected in GF‐perio secretomes, including several growth factors, cytokines, chemokines, and extracellular matrix proteins important for wound healing and regeneration. Key bone‐related cytokines (FGF2, MCP1, GPNMB, MMP2, IL6, IL8) were confirmed by an immunoassay. Compared to the reference group, 127 exclusive proteins and 73 DEPs (p < 0.05) were identified in the GF‐perio group. FEA revealed significant enrichment of “exosome” and “cytoplasm” related cellular components in GF‐perio secretomes. Conclusion The secretome of GF from periodontally diseased tissues may hold therapeutic potential, with several proteins important for wound healing and regeneration, especially those related to exosome functions.