PGK1 can affect the prognosis and development of bladder cancer

• Published in: Cancer medicine (Malden, MA) Vol. 13; no. 18; pp. e70242 - n/a
• Main Authors: Gao, Mingde, Zhu, Haixia, Xu, Haifei, Jin, Xiaoxia, Zheng, Guihua, Zhu, Jinfeng, Gu, Chunyan, Wang, Xiaolin
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.09.2024; John Wiley and Sons Inc; Wiley
• Subjects: Aged; Antibodies; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Bladder cancer; Cell Line, Tumor; Cell migration; Cell Movement; Cell Proliferation; chemosensitivity; Chemotherapy; Cholecystokinin; Cisplatin; Cisplatin - pharmacology; Cisplatin - therapeutic use; Enzymes; Female; Gene expression; Gene Expression Regulation, Neoplastic; Glycolysis; Humans; Immunohistochemistry; Kinases; Liver cancer; Lymphatic system; Male; Medical prognosis; Metastases; Metastasis; Middle Aged; mRNA; Patients; PGK1; Phosphoglycerate kinase; Phosphoglycerate Kinase - genetics; Phosphoglycerate Kinase - metabolism; Phosphoglycerate kinase 1; Prognosis; proliferation; Protein expression; Proteins; Regression analysis; Software; Statistical analysis; Urinary Bladder Neoplasms - drug therapy; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - mortality; Urinary Bladder Neoplasms - pathology; Urological surgery; PGK1; proliferation; chemosensitivity; metastasis; prognosis; bladder cancer
• ISSN: 2045-7634; 2045-7634
• DOI: 10.1002/cam4.70242

Background Previous studies have demonstrated that the glycolytic enzyme phosphoglycerate kinase 1 (PGK1) can promote tumor development. This study sought to investigate the specific role of PGK1 in bladder cancer (BLCA). Methods Public databases and immunohistochemistry assays were utilized to analyze the expression of PGK1 in BLCA and its prognostic significance. Cell proliferation was assessed through CCK‐8 and colony formation assays, while the level of metastasis was evaluated using transwell migration experiments. Additionally, IC50 experiments were conducted to assess the impact of PGK1 on cisplatin sensitivity. Results The mRNA and protein expression levels of PGK1 were significantly upregulated in BLCA. Cox proportional hazards model analysis revealed that PGK1 and T stage were independent prognostic factors for BLCA patients. Both CCK‐8 and colony assays demonstrated that PGK1 promotes proliferation. Furthermore, a positive correlation was observed between PGK1 and Ki67, a proliferation index. Transwell migration assays confirmed the ability of PGK1 to enhance metastasis. Finally, PGK1 increased the IC50 associated with cisplatin treatment in BLCA. Conclusion Collectively, these findings suggest that PGK1 may hold clinical value in predicting BLCA prognosis and improving the outcomes of this patient population.