Structure of the O-specific polysaccharide of Proteus penneri strain 41 from a new proposed serogroup O62

• Published in: FEMS immunology and medical microbiology Vol. 21; no. 1; pp. 1 - 9
• Main Authors: Zych, Krystyna, Knirel, Yuriy A., Paramonov, Nikolay A., Vinogradov, Evgeny V., Arbatsky, Nikolay P., Senchenkova, Sofya N., Shashkov, Alexander S., Sidorczyk, Zygmunt
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.05.1998; Oxford University Press
• Subjects: 2‐Acetamido‐4‐O‐[(S)‐1‐carboxyethyl]‐2‐deoxy‐d‐glucose; Animals; Carbohydrate Conformation; Carbohydrate Sequence; Cross Reactions - immunology; Cross-reactivity; Deoxyglucose; Lipopolysaccharide; Magnetic resonance spectroscopy; Molecular Sequence Data; NMR; NMR spectroscopy; Nuclear magnetic resonance; Nuclear Magnetic Resonance, Biomolecular; O Antigens - chemistry; O Antigens - immunology; O Antigens - isolation & purification; O‐specific polysaccharide; Polysaccharides; Proteus - chemistry; Proteus - classification; Proteus - immunology; Proteus penneri; Rabbits; Serotyping; Spectroscopy; Spectrum analysis; Lipopolysaccharide; O-specific polysaccharide; glucose; -; 2-Acetamido-4; 1-carboxyethyl]-2-deoxy
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.1998.tb01143.x

Abstract O-specific polysaccharide of Proteus penneri strain 41 was studied using 1H- and 13C-NMR spectroscopy, including two-dimensional COSY, heteronuclear 13C,1H-correlation (HETCOR) and one-dimensional NOE spectroscopy, and the following structure of a non-stoichiometrically O-acetylated hexasaccharide repeating unit was established: where RGlcNAc is 2-acetamido-4-O-[(S)-1-carboxyethyl]-2-deoxyglucose. Cross-reactivity of anti-P. penneri 41 O-serum with other P. penneri strains is discussed, and a new, separate O62 serogroup is proposed which is the next Proteus O-serogroup containing P. penneri strains only.