Soluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth

• Published in: FEMS microbiology letters Vol. 165; no. 1; pp. 111 - 116
• Main Authors: Arikawa, Yukihiko, Enomoto, Keiichiro, Muratsubaki, Haruhiro, Okazaki, Mitsuo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.1998; Blackwell; Oxford University Press
• Subjects: Anaerobic conditions; Anaerobiosis; Biological and medical sciences; Carbon sources; Cloning, Molecular; Disruption; Fumarate reductase; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; Fungal Proteins - metabolism; Genes; Genotype; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Isoenzymes; Isoenzymes - metabolism; Microbiology; Mycology; OSM1 gene; Promitochondrial enzyme; Reductase; Reductases; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae Proteins; Succinate Dehydrogenase - genetics; Succinate Dehydrogenase - metabolism; Time Factors; Yeast; Yeasts; Yeast; Fumarate reductase; gene; Promitochondrial enzyme; Anaerobiosis; Microorganism growth; Succinate dehydrogenase; Osmolarity; Enzyme; Isozyme; Environmental factor; Metabolism; Fungi; Gene; Ascomycetes; Oxidoreductases; Osmotic pressure; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1998.tb13134.x

Abstract In Saccharomyces cerevisiae, the cytosolic and promitochondrial isoenzymes of fumarate reductase are encoded by the FRDS and OSM1 genes, respectively. The product of the OSM1 gene is reported to be required for growth in hypertonic medium. Simultaneous disruption of the FRDS and OSM1 genes resulted in the inability of the yeasts to grow anaerobically on glucose as a carbon source, and disruption of the OSM1 gene caused poor growth under anaerobic conditions. However, the disruption of both the FRDS and/or OSM1 genes had no effect on aerobic growth or growth under hypertonic conditions. These results suggest that the fumarate reductase isoenzymes in Saccharomyces cerevisiae are essential for anaerobic growth but not for growth under hypertonic conditions.