A new type of capillary column for open-tubular electrochromatography
A new type of open‐tubular C18 ester‐bonded electrochromatographic column was prepared with sol‐gel technology, followed by an on‐column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol‐gel precursor to form a thin coating...
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Published in | Electrophoresis Vol. 23; no. 17; pp. 2990 - 2995 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
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Weinheim
WILEY-VCH Verlag
01.09.2002
WILEY‐VCH Verlag |
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Abstract | A new type of open‐tubular C18 ester‐bonded electrochromatographic column was prepared with sol‐gel technology, followed by an on‐column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol‐gel precursor to form a thin coating layer on the wall of the fused‐silica capillary. The C18 groups were introduced into the coating layer by on‐column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8×105 plates/m was achieved for a basic pentapeptide using the C18 ester‐bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol‐gel‐coated capillaries, the C18 ester‐bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage. |
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AbstractList | A new type of open‐tubular C18 ester‐bonded electrochromatographic column was prepared with sol‐gel technology, followed by an on‐column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol‐gel precursor to form a thin coating layer on the wall of the fused‐silica capillary. The C18 groups were introduced into the coating layer by on‐column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8×105 plates/m was achieved for a basic pentapeptide using the C18 ester‐bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol‐gel‐coated capillaries, the C18 ester‐bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage. A new type of open-tubular C(18) ester-bonded electrochromatographic column was prepared with sol-gel technology, followed by an on-column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol-gel precursor to form a thin coating layer on the wall of the fused-silica capillary. The C(18) groups were introduced into the coating layer by on-column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8x10(5) plates/m was achieved for a basic pentapeptide using the C(18 )ester-bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol-gel-coated capillaries, the C(18) ester-bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage. |
Author | Liu, Guoquan Shangguan, Dihua Zhao, Rui Zhao, Yingxin |
Author_xml | – sequence: 1 givenname: Yingxin surname: Zhao fullname: Zhao, Yingxin organization: Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China – sequence: 2 givenname: Rui surname: Zhao fullname: Zhao, Rui organization: Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China – sequence: 3 givenname: Dihua surname: Shangguan fullname: Shangguan, Dihua organization: Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China – sequence: 4 givenname: Guoquan surname: Liu fullname: Liu, Guoquan email: liugq@infoc3.icas.ac.cn. organization: Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China |
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Cites_doi | 10.1016/S0021-9673(97)00181-7 10.1016/S0021-9673(99)00154-5 10.1021/ac9717245 10.1016/S0021-9673(99)01295-9 10.1016/0021-9673(95)01320-2 10.1021/ac960894z 10.1021/ac00111a003 10.1016/S0021-9673(00)95263-4 10.1007/BF02535730 |
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References_xml | – volume: 186 start-page: 405 year: 1979 end-page: 418 publication-title: J. Chromatogr. – volume: 69 start-page: 461A year: 1997 end-page: 467A publication-title: Anal. Chem. – volume: 69 start-page: 581 year: 1997 end-page: 586 publication-title: Anal. Chem. – volume: 736 start-page: 255 year: 1996 end-page: 264 publication-title: J. Chromatogr. A – volume: 67 start-page: 2511 year: 1995 end-page: 2516 publication-title: Anal. Chem. – volume: 845 start-page: 237 year: 1999 end-page: 246 publication-title: J. Chromatogr. A – volume: 52 start-page: 517 year: 2000 end-page: 519 publication-title: Chromatographia – volume: 773 start-page: 65 year: 1997 end-page: 72 publication-title: J. Chromatogr. A – volume: 892 start-page: 257 year: 2000 end-page: 278 publication-title: J. Chromatogr. A – volume: 773 start-page: 65 year: 1997 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB8 publication-title: J. Chromatogr. A doi: 10.1016/S0021-9673(97)00181-7 contributor: fullname: Narang – volume: 845 start-page: 237 year: 1999 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB6 publication-title: J. Chromatogr. A doi: 10.1016/S0021-9673(99)00154-5 contributor: fullname: Pesek – volume: 69 start-page: 461a year: 1997 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB1 publication-title: Anal. Chem. doi: 10.1021/ac9717245 contributor: fullname: Colón – volume: 892 start-page: 257 year: 2000 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB2 publication-title: J. Chromatogr. A doi: 10.1016/S0021-9673(99)01295-9 contributor: fullname: Pyell – volume: 736 start-page: 255 year: 1996 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB5 publication-title: J. Chromatogr. A doi: 10.1016/0021-9673(95)01320-2 contributor: fullname: Pesek – volume: 69 start-page: 581 year: 1997 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB4 publication-title: Anal. Chem. doi: 10.1021/ac960894z contributor: fullname: Tan – volume: 67 start-page: 2511 year: 1995 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB7 publication-title: Anal. Chem. doi: 10.1021/ac00111a003 contributor: fullname: Guo – volume: 186 start-page: 405 year: 1979 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB3 publication-title: J. Chromatogr. doi: 10.1016/S0021-9673(00)95263-4 contributor: fullname: Knox – volume: 52 start-page: 517 year: 2000 ident: 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7-BIB9 publication-title: Chromatographia doi: 10.1007/BF02535730 contributor: fullname: Yu |
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Snippet | A new type of open‐tubular C18 ester‐bonded electrochromatographic column was prepared with sol‐gel technology, followed by an on‐column octadecyl silylation... A new type of open-tubular C(18) ester-bonded electrochromatographic column was prepared with sol-gel technology, followed by an on-column octadecyl silylation... |
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SubjectTerms | Chromatography - instrumentation Chromatography - methods Electrophoresis, Capillary - instrumentation Electrophoresis, Capillary - methods Indicators and Reagents Open-tubular capillary electrochromatography Peptides Reproducibility of Results Silanes - chemical synthesis Silanes - chemistry Sol-gel technology |
Title | A new type of capillary column for open-tubular electrochromatography |
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