A new type of capillary column for open-tubular electrochromatography
A new type of open‐tubular C18 ester‐bonded electrochromatographic column was prepared with sol‐gel technology, followed by an on‐column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol‐gel precursor to form a thin coating...
Saved in:
Published in | Electrophoresis Vol. 23; no. 17; pp. 2990 - 2995 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.09.2002
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | A new type of open‐tubular C18 ester‐bonded electrochromatographic column was prepared with sol‐gel technology, followed by an on‐column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol‐gel precursor to form a thin coating layer on the wall of the fused‐silica capillary. The C18 groups were introduced into the coating layer by on‐column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8×105 plates/m was achieved for a basic pentapeptide using the C18 ester‐bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol‐gel‐coated capillaries, the C18 ester‐bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage. |
---|---|
Bibliography: | ark:/67375/WNG-LZP9SPNW-H istex:A7C8350AE59D50F7E809AFD5B6DB0EE5FBEA30E1 ArticleID:ELPS2990 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/1522-2683(200209)23:17<2990::AID-ELPS2990>3.0.CO;2-7 |