Phylogenetic relationships of the genus Kluyvera: transfer of Enterobacter intermedius Izard et al. 1980 to the genus Kluyvera as Kluyvera intermedia comb. nov. and reclassification of Kluyvera cochleae as a later synonym of K. intermedia

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 1; pp. 437 - 442
• Main Authors: Pavan, Maria E, Franco, Raul J, Rodriguez, Juan M, Gadaleta, Patricia, Abbott, Sharon L, Janda, J. Michael, Zorzopulos, Jorge
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.01.2005; Society for General Microbiology
• Subjects: Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Enterobacter; Enterobacter - classification; Enterobacter - genetics; Enterobacter - metabolism; Enterobacter intermedius; Enterobacteriaceae; Enterobacteriaceae - classification; Enterobacteriaceae - genetics; Enterobacteriaceae - metabolism; Fundamental and applied biological sciences. Psychology; Kluyvera; Kluyvera - classification; Kluyvera - genetics; Kluyvera - metabolism; Kluyvera cochleae; Kluyvera cryocrescens; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phenotype; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; Terminology as Topic; Taxonomic review; Enterobacter; Kluyvera; Taxonomy; Synonymy; Bacteria; Phylogeny; Enterobacteriaceae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63071-0

1 Instituto de Investigaciones Biomédicas Fundación Pablo Cassará, Saladillo 2452, Buenos Aires (1440), Argentina 2 Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina 3 Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Richmond, CA, USA Correspondence Jorge Zorzopulos zorzopul{at}hotmail.com In order to assess the relationship between the genus Kluyvera and other members of the family Enterobacteriaceae , the 16S rRNA genes of type strains of the recognized Kluyvera species, Kluyvera georgiana , Kluyvera cochleae , Kluyvera ascorbata and Kluyvera cryocrescens , were sequenced. A comparative phylogenetic analysis based on these 16S rRNA gene sequences and those available for strains belonging to several genera of the family Enterobacteriaceae showed that members of the genus Kluyvera form a cluster that contains all the known Kluyvera species. However, the type strain of Enterobacter intermedius (ATCC 33110 T ) was included within this cluster in a very close relationship with the type strain of K. cochleae (ATCC 51609 T ). In addition to the phylogenetic evidence, biochemical and DNA–DNA hybridization analyses of species within this cluster indicated that the type strain of E. intermedius is in fact a member of the genus Kluyvera and, within it, of the species Kluyvera cochleae . Therefore, following the current rules for bacterial nomenclature and classification, the transfer of E. intermedius to the genus Kluyvera as Kluyvera intermedia comb. nov. is proposed (type strain, ATCC 33110 T =CIP 79.27 T =LMG 2785 T =CCUG 14183 T ). Biochemical analysis of four E. intermedius strains and one K. cochleae strain independent of the respective type strains further indicated that E. intermedius and K. cochleae represent the same species and are therefore heterotypic synonyms. Nomenclatural priority goes to the oldest legitimate epithet. Consequently, Kluyvera cochleae Müller et al . 1996 is a later synonym of Kluyvera intermedia (Izard et al . 1980) Pavan et al . 2005. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of K. cochleae ATCC 51609 T , K. georgiana ATCC 51603 T , K. cryocrescens ATCC 33435 T , K. ascorbata ATCC 33433 T and Enterobacter intermedius ATCC 33110 T are AF047187 , AF047186 , AF310218 , AF310219 and AF310217 , respectively. A phylogenetic tree showing that members of the genus Enterobacter are intertwined with members of other genera is available as supplementary material in IJSEM Online.