Experimental Correction of Homeostasis Changes during Alloxan-Induced Diabetes by Implantation of Islet Cells Cultured in Fibrous TiNi-Based Scaffold

• Published in: Bulletin of experimental biology and medicine Vol. 174; no. 1; pp. 89 - 94
• Main Authors: Kokorev, O. V., Khlusov, I. A., Marchenko, E. S., Yasenchuk, Yu. F., Dambaev, G. Ts
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2022; Springer; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Blood sugar; Cell Biology; Diabetes; Diabetes mellitus; Ethylenediaminetetraacetic acid; Experimental Methods for Clinical Practice; Glucose metabolism; Glycosylated hemoglobin; Hemoglobin; Hepatocytes; Homeostasis; Internal Medicine; Islet cells; Laboratory Medicine; Life span; Pathology; Pyrimidines; Scanning electron microscopy; Tissue engineering; Titanium; hybrid scaffold; biochemical and hematological parameters; lifespan of animals; rats; biocompatibility
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-022-05654-5

The use of fibrous scaffolds made of titanium nickelide and carrying islet cells (IC) after a course of alloxan administration was studied in vivo . Scanning electron microscopy showed good adhesion of IC in the pore space of the scaffolds. In 28 days, mature tissue comprising both cellular and fibrous components filled the inner space of the scaffold by 90%. The advantage of intraperitoneal implantation of IC in vitro seeded in fibrous titanium nickelide scaffolds in comparison with injection of IC (6×10 5 cells) to Wistar rats was demonstrated in the dynamics of pancreatic function recovery (normalization of blood glucose and glycosylated hemoglobin concentrations by day 21 of the experiment), regeneration of the white hematopoietic lineage cells, and lengthening (by 2 times) of the lifespan of animals with alloxan-induced diabetes. Injection of IC improved rat survival by only 40%. Thus, the hybrid tissue-engineered construct (fibrous titanium nickelide scaffold+IC) is biocompatible when administered intraperitoneally, stimulates regeneration in rats with alloxan-induced diabetes, improves blood glucose utilization processes, and restores suppressed leukopoiesis. It is assumed that hepatocytes can be the main target cells of the hybrid tissue-engineering construct.