Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR

We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the interaction of the RNA aptamer with argininamide in living human cells with...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 59; no. 1; pp. 12 - 15
Main Authors Eladl, Omar, Yamaoki, Yudai, Kondo, Keiko, Nagata, Takashi, Katahira, Masato
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 22.12.2022
Subjects
Aptamers, Nucleotide - chemistry
E coli
Escherichia coli
HeLa Cells
Humans
Magnetic Resonance Spectroscopy
NMR
Nuclear magnetic resonance
tat Gene Products, Human Immunodeficiency Virus
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Summary:We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the interaction of the RNA aptamer with argininamide in living human cells with higher resolution. A labeled RNA aptamer prepared with E. coli was introduced into human cells. Aptamer interaction was investigated using 2D in-cell NMR.
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https://doi.org/10.1039/d2cc05576g
H-
C HMQC full spectra of TA_36. Materials and methods. See DOI
Electronic supplementary information (ESI) available: (Fig. S1) Imino proton spectrum of an RNA aptamer for HIV-1 Tat (TA_36) at each molar ratio of [(TA_36]:[argininamide] acquired in transport buffer (TB). (Fig. S2) 2D in-cell
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ISSN:1359-7345
1364-548X
DOI:10.1039/d2cc05576g