Prognostic Value of microRNA Signature in Patients with Gastric Cancers

• Published in: Scientific reports Vol. 7; no. 1; p. 42806
• Main Authors: Liu, Hai-Ting, Wang, Ya-Wen, Xing, Ai-Yan, Shi, Duan-Bo, Zhang, Hui, Guo, Xiang-Yu, Xu, Jing, Gao, Peng
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 16.02.2017
• Subjects: Female; Gastric cancer; Gene Expression Profiling - methods; Gene Expression Regulation, Neoplastic; Humans; Logistic Models; Lymph; Lymph nodes; Lymphatic Metastasis - genetics; Male; Medical prognosis; Metastases; MicroRNAs - genetics; miRNA; Polymerase chain reaction; Prognosis; Stomach Neoplasms - genetics; Survival; Survival Analysis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep42806

The occurrence of lymph node metastases (LNM) after endoscopic submucosal dissection (ESD) in patients with gastric cancer (GC) leads to poor prognosis. However, few biomarkers are available to predict LNM in GC patients. Thus, we measured expression of 6 cancer-related miRNAs using real-time RT-PCR in 102 GC samples that were randomized into a training set and a testing set (each, 51 cases). Using logistic regression, we identified 4-miRNA (miR-27b, miR-128, miR-100 and miR-214) signatures for predicting LNM in GC patients. Patients with high-risk scores for the 4-miRNA signature tended to have higher LNM than those with low-risk scores. Meanwhile, the ROC curve of the 4-miRNA signature was better for predicting LNM in GC patients. In addition, Cox regression analysis indicated that a 2-miRNA signature (miR-27b and miR-214) or a miR-214/N stage signature was predictive of survival for GC patients. This work describes a previously unrecognized 4-miRNA signature involved in LNM and a 2-miRNA signature or miR-214/N stage signature related to GC patients' survival.