Enzymatic production of 5-aminovalerate from L-lysine using L-lysine monooxygenase and 5-aminovaleramide amidohydrolase

5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It is a metabolite of l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. L-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase...

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Published inScientific reports Vol. 4; no. 1; p. 5657
Main Authors Liu, Pan, Zhang, Haiwei, Lv, Min, Hu, Mandong, Li, Zhong, Gao, Chao, Xu, Ping, Ma, Cuiqing
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 11.07.2014
Subjects
Amidohydrolases - metabolism
Amino Acids, Neutral - metabolism
Biotransformation
E coli
Enzymes
Fermentation
Fermentation - physiology
Laboratories
Lysine
Lysine - metabolism
Mixed Function Oxygenases - metabolism
Monooxygenase
Oxidation
Pseudomonas putida - metabolism
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Summary:5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It is a metabolite of l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. L-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA) play key roles in the biotransformation of L-lysine into 5-aminovalerate. Here, DavB and DavA of P. putida KT2440 were expressed, purified, and coupled for the production of 5-aminovalerate from L-lysine. Under optimal conditions, 20.8 g/L 5-aminovalerate was produced from 30 g/L L-lysine in 12 h. Because L-lysine is an industrial fermentation product, the two-enzyme coupled system presents a promising alternative for the production of 5-aminovalerate.
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ISSN:2045-2322
2045-2322
DOI:10.1038/srep05657