Molecular cloning and sequencing of a carp cDNA encoding mitogen-activated protein kinase kinase

We have cloned a full-length cDNA encoding the mitogen-activated protein kinase kinase (MKK) from a carp liver cDNA library. The cDNA contains 1970 bp with a single open reading frame encoding a 397 amino acid protein. By comparing with known MKK sequences from other species, carp MKK is 78%, 80%, 7...

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Published inBiochimica et biophysica acta Vol. 1220; no. 2; pp. 223 - 225
Main Authors Huang, Chang-Jen, Lee, Ming-Shyue, Chang, Geen-Dong, Huang, Fore-Lien, Lo, Tung-Bin
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 13.01.1994
Subjects
Amino Acid Sequence
Animals
Base Sequence
C. carpio
Carp
Carps - genetics
Cloning, Molecular
DNA, Complementary - chemistry
Enzyme Activation - drug effects
MAP kinase kinase
MAP Kinase Kinase 1
MAP Kinase Kinase 2
Mitogen-Activated Protein Kinase Kinases
Mitogens - pharmacology
Molecular Sequence Data
Polymerase chain reaction
Protein-Serine-Threonine Kinases - genetics
Protein-Tyrosine Kinases - genetics
Recombinant DNA
Sequence Alignment
Polymerase chain reaction
Carp
Recombinant DNA
C. carpio
MAP kinase kinase
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Summary:We have cloned a full-length cDNA encoding the mitogen-activated protein kinase kinase (MKK) from a carp liver cDNA library. The cDNA contains 1970 bp with a single open reading frame encoding a 397 amino acid protein. By comparing with known MKK sequences from other species, carp MKK is 78%, 80%, 76% and 58% identical to rat MKK1, rat MKK2, Xenopus MKK and Drosophila MKK.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0167-4889
0006-3002
1879-2596
DOI:10.1016/0167-4889(94)90140-6