Identification of a promoter sequence in the plasmid pUL340 of Brevibacterium lactofermentum and construction of new cloning vectors for corynebacteria containing two selectable markers

• Published in: Gene Vol. 56; no. 2; pp. 199 - 208
• Main Authors: Santamaría, R.I., Martin, J.F., Gil, J.A.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 1987; Amsterdam Elsevier; New York, NY
• Subjects: Applied microbiology; Biological and medical sciences; Biotechnology; Brevibacterium - genetics; chloramphenicol; Chromosome Mapping; Cloning, Molecular; Corynebacterium - genetics; Drug Resistance, Microbial; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic Markers; Genetic technics; Genetic Vectors; hygromycin; kanamycin; Methods. Procedures. Technologies; Microbiology; Miscellaneous; Plasmids; Promoter Regions, Genetic; Recombinant DNA; Streptomyces - genetics; Synthetic digonucleotides and genes. Sequencing; Tn 5 transposon; HPH; Km; Cm; Recombinant DNA; bp; Ap; Tc; bla; Hyg; kb; Tn; aa; MM; tet; TSA; kanamycin; vph; r; PEG; s; LA; CAT; Tn 5 transposon; hygromycin; chloramphenicol; Resistance; Antibiotic; Gene; Brevibacterium lactofermentum; Transcription promoter; Brevibacteriaceae; Bacteria; Chloramphenicol; Actinomycetes; Molecular cloning; Vector; Kanamycine A
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(87)90137-5

A strong promoter P1 has been found in plasmid pUL340, a cloning vector used to transform corynebacteria. This promoter is also expressed efficiently in Escherichia coli. A gene ( cat) for chloramphenicol acetyltransferase from Streptomyces acrimycini and a gene ( hyg) for hygromycin phosphotransferase from Streptomyces hygroscopicus were subcloned in different positions of the Brevibacterium lactofermentum plasmid pUL340. Both resistance genes are expressed in B. lactofermentum from their own promoters or from the endogenous promoter in pUL340. These genes provide useful screening markers for selecting transformants of B. lactofermentum together with the kanamycin-resistance gene from the transposon Tn 5.