Isolation and characterization of proline/betaine transporter gene from oil palm

• Published in: Tree physiology Vol. 31; no. 4; pp. 462 - 468
• Main Authors: Yamada, Nana, Cha-Um, Suriyan, Kageyama, Hakuto, Promden, Worrawat, Tanaka, Yoshito, Kirdmanee, Chalermpol, Takabe, Teruhiro
• Format: Journal Article
• Language: English
• Published: Canada 01.04.2011
• Subjects: Amino Acid Transport Systems, Neutral - drug effects; Amino Acid Transport Systems, Neutral - genetics; Amino Acid Transport Systems, Neutral - metabolism; Arecaceae - drug effects; Arecaceae - genetics; Arecaceae - metabolism; Base Sequence; Betaine - analysis; Betaine - metabolism; Biological Transport; Carrier Proteins - drug effects; Carrier Proteins - genetics; Carrier Proteins - metabolism; Choline - metabolism; Cloning, Molecular; DNA, Complementary - chemistry; DNA, Complementary - genetics; DNA, Plant - chemistry; DNA, Plant - genetics; Green Fluorescent Proteins; Molecular Sequence Data; Phylogeny; Proline - analysis; Proline - metabolism; Promoter Regions, Genetic - genetics; RNA, Messenger - genetics; RNA, Plant - genetics; Seeds - genetics; Seeds - metabolism; Sequence Analysis, DNA; Sodium Chloride - pharmacology; Stress, Physiological
• ISSN: 0829-318X; 1758-4469
• DOI: 10.1093/treephys/tpr017

Oil production from oil palm is adversely affected by drought and salt. Under drought and salt stress, proline content increases in oil palm; the mechanism for this is unknown. Here, an 8319-nucleotide sequence including cDNA, genomic DNA and the promoter region of proline transporter gene from oil palm Elaeis guineensis was determined. The transporter gene exhibited high similarity to Bet/ProT genes from several plants, but the highest homology was found with rice ProT1. The exon-intron structure of genomic DNA was unique, and numerous stress-response cis-elements were found in the promoter region. Expression of cDNA EgProT1 in Escherichia coli mutant exhibited uptake activities for glycinebetaine and choline as well as proline. Under salt-stressed conditions, exogenously applied glycinebetaine was taken up into the root more rapidly than the control. These data indicate that oil palm has a unique Pro/T1 gene. Nucleotide sequence data for the cDNA and genomic DNA of proline transporter gene from Elaeis guineensis are available in the DDJB database under accession numbers AB597035 and AB597036, respectively.