A functionalized dumbbell probe-based cascading exponential amplification DNA machine enables amplified probing of microRNAs

A functionalized dumbbell probe (FDP) based amplification method, termed as a cascading exponential amplification DNA machine (CEA-DNA machine), has been developed to autonomously accumulate single G-quadruplexes (SGQs) and twin-G-quadruplexes (TGQs) for robust fluorescence signal-on probing of miRN...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 56; no. 11; pp. 1681 - 1684
Main Authors Wang, Jie, Li, Shuhui, Xu, Jianguo, Lu, Yusheng, Lin, Min, Wang, Chaihung, Zhang, Chen, Lin, Guoxing, Jia, Lee
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 06.02.2020
Subjects
Amplification
Benzothiazoles - chemistry
Biosensing Techniques - methods
Cell Line, Tumor
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - genetics
DNA Probes - chemistry
DNA Probes - genetics
Fluorescence
Fluorescent Dyes - chemistry
G-Quadruplexes
Humans
Inverted Repeat Sequences
Limit of Detection
MicroRNAs
MicroRNAs - blood
MicroRNAs - genetics
Nucleic Acid Amplification Techniques - methods
Nucleic Acid Hybridization
Spectrometry, Fluorescence - methods
Online AccessGet full text

Cover

More Information
Summary:A functionalized dumbbell probe (FDP) based amplification method, termed as a cascading exponential amplification DNA machine (CEA-DNA machine), has been developed to autonomously accumulate single G-quadruplexes (SGQs) and twin-G-quadruplexes (TGQs) for robust fluorescence signal-on probing of miRNA-21. A functionalized dumbbell probe-based cascading exponential amplification DNA machine has been developed for robust determination of miRNA-21.
Bibliography:10.1039/c9cc08289a
Electronic supplementary information (ESI) available. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc08289a