Enzymatic production of N-acetyl- d-glucosamine from chitin. Degradation study of N-acetylchitooligosaccharide and the effect of mixing of crude enzymes

N-Acetyl- d-glucosamine (GlcNAc) was produced from chitin by use of crude enzyme preparations. The efficient production of GlcNAc by cellulases derived from Trichoderma viride (T) and Acremonium cellulolyticus (A) was observed by HPLC analysis compared to lipase, hemicellulase, and pectinase. β-Chit...

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Published inCarbohydrate polymers Vol. 51; no. 4; pp. 391 - 395
Main Authors Sashiwa, Hitoshi, Fujishima, Shizu, Yamano, Naoko, Kawasaki, Norioki, Nakayama, Atsuyoshi, Muraki, Einosuke, Sukwattanasinitt, Mongkol, Pichyangkura, Rath, Aiba, Sei-ichi
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.03.2003
Elsevier Science
Subjects
Applied sciences
Chitin
Crude enzyme
Exact sciences and technology
N-Acetyl- d-glucosamine
Natural polymers
Physicochemistry of polymers
Starch and polysaccharides
N-Acetyl- d-glucosamine
Crude enzyme
Chitin
Depolymerization
Hydrolysis
Aminoglycoside
N-Acetyl-o-glucosamine
Enzymatic digestion
Oside polymer
Experimental study
Optimization
Operating conditions
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Summary:N-Acetyl- d-glucosamine (GlcNAc) was produced from chitin by use of crude enzyme preparations. The efficient production of GlcNAc by cellulases derived from Trichoderma viride (T) and Acremonium cellulolyticus (A) was observed by HPLC analysis compared to lipase, hemicellulase, and pectinase. β-Chitin showed higher degradability than α-chitin when using cellulase T. The optimum pH of cellulase T was 4.0 on the hydrolysis of β-chitin. The yield of GlcNAc was enhanced by mixing of cellulase T and A.
ISSN:0144-8617
1879-1344
DOI:10.1016/S0144-8617(02)00192-3